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The prevalence and predictive value of the SLC30A8 R325W polymorphism and zinc transporter 8 autoantibodies in the development of GDM and postpartum type 1 diabetes

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Abstract

The objectives were to evaluate possible associations between the SLC30A8 R325W polymorphism and gestational diabetes mellitus (GDM) as well as postpartum development of type 2 diabetes. Furthermore, we wanted to confirm the prevalence of zinc transporter 8 autoantibodies (ZnT8A), as previously reported, in a larger population and study its predictive value in relation to other β cell specific autoantibodies in postpartum development of type 1 diabetes. Women diagnosed with GDM (n = 776) and women without diabetes (n = 511) were included in the study. Autoantibodies were analyzed in all women using enzyme-linked immunosorbent assay. DNA was extracted when possible from women with GDM (n = 536) and all of the controls. R325W was detected through polymerase chain reaction and specific restriction digestion. The R325W C-allele were more frequent in women with GDM compared to in controls (OR 1.47, 95 % CI 1.16–1.88, p = 0.0018) but not significantly increased in women with GDM and postpartum development of type 2 diabetes. Autoantibodies were found in 6.8 % (53/776) of the women with GDM and approximately 3.2 % (25/776) were ZnT8A positive. Approximately 19 % (10/53) of the autoantibody positive women with GDM developed postpartum type 1 diabetes. In conclusion, this is the first study to report a significant association between the R325W C-allele and increased risk of developing GDM. All of the autoantibody positive women with GDM who developed postpartum type 1 diabetes were positive for autoantibodies against glutamic acid decarboxylase (GADA). Thus ZnT8A did not have any additional predictive value in postpartum development of type 1 diabetes.

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Acknowledgments

The authors would like to thank Mrs. Birgitte Ekholm for her excellent technical assistance in the research laboratory. The authors would also like to thank Carl and Erik Olsson for their invaluable help with the extraction of DNA. This study was supported by grants from the Swedish Medical Research Council and the Swedish Diabetes Foundation.

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Authors and Affiliations

  1. Department of Clinical Sciences, Diabetes Research Laboratory, Lund University, B11, BMC, 221 84, Lund, Sweden

    Jonatan Dereke, Sanna Palmqvist, Charlotta Nilsson, Mona Landin–Olsson & Magnus Hillman

  2. Department of Pediatrics, Helsingborg Hospital, Helsingborg, Sweden

    Charlotta Nilsson

  3. Department of Endocrinology, Skåne University Hospital, Lund, Sweden

    Mona Landin–Olsson

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  1. Jonatan Dereke
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  2. Sanna Palmqvist
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Correspondence to Jonatan Dereke.

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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Dereke, J., Palmqvist, S., Nilsson, C. et al. The prevalence and predictive value of the SLC30A8 R325W polymorphism and zinc transporter 8 autoantibodies in the development of GDM and postpartum type 1 diabetes. Endocrine 53, 740–746 (2016). https://doi.org/10.1007/s12020-016-0932-7

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