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Evaluation of protein oxidation and its association with lipid peroxidation and thyrotropin levels in overt and subclinical hypothyroidism

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Abstract

Both overt (OHT) and subclinical hypothyroid (SHT) disorders have been found to be associated with increased oxidative stress (OXS). Excess thyrotropin [thyroid stimulating hormone (TSH)] is known to directly produce OXS. Increased lipid peroxidation is known to facilitate protein carbonylation. However, the associations between lipid and protein oxidation and elevated TSH levels have not been studied. Thyroid profile, lipid peroxidation as malondialdehyde (MDA) levels and protein carbonylation as protein carbonyls (PCO) were estimated in OHT and SHT groups consisting of 36 patients each, in comparison to 39 euthyroid controls. We also determined the associations between TSH, MDA, and PCO levels in OHT and SHT groups. Increased oxidative damage was evidenced through significant elevations in the concentrations of MDA and PCO in OHT and SHT groups compared to controls (p < 0.01). Both TSH and MDA levels were positively associated with PCO in OHT group. Partial correlation analysis revealed that both excess TSH and increased MDA levels are mutually influencing elevated PCO. The results indicate that there is a simultaneous oxidative damage to lipids and proteins leading to increased MDA and PCO levels in both patient groups. Either of the excess TSH and increased MDA levels are combinably involved in the elevation of PCO in hypothyroidism.

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Abbreviations

OHT:

Overt hypothyroidism

SHT:

Subclinical hypothyroidism

OXS:

Oxidative stress

TSH:

Thyroid stimulating hormone (thyrotropin)

MDA:

Malondialdehyde

PCO:

Protein carbonyls

ROS:

Reactive oxygen species

DNA:

Deoxyribonucleic acid

T3:

Tri-iodothyronine

T4:

Tetra-iodothyronine

RIA:

Radioimmunoassay

IRMA:

Immunoradiometric assay

DNPH:

Di-nitrophenyl hydrazine

ANOVA:

Analysis of variance

ROC:

Receiver operating characteristics curve

AUC:

Area under curve

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Biochemistry, Sri Venkateswara Institute of Medical Sciences (SVIMS), Tirupati, 517507, Andhra Pradesh, India

    A. Haribabu, V. Seshadri Reddy, Ch. Pallavi, Aparna R. Bitla, P. Pullaiah, P. V. L. N. Srinivasa Rao & M. M. Suchitra

  2. Department of Endocrinology, Sri Venkateswara Institute of Medical Sciences, Tirupati, 517507, Andhra Pradesh, India

    Alok Sachan & V. Suresh

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  1. A. Haribabu
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  2. V. Seshadri Reddy
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  3. Ch. Pallavi
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  6. P. Pullaiah
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  8. P. V. L. N. Srinivasa Rao
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  9. M. M. Suchitra
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Correspondence to M. M. Suchitra.

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Haribabu, A., Reddy, V.S., Pallavi, C. et al. Evaluation of protein oxidation and its association with lipid peroxidation and thyrotropin levels in overt and subclinical hypothyroidism. Endocrine 44, 152–157 (2013). https://doi.org/10.1007/s12020-012-9849-y

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  • DOI: https://doi.org/10.1007/s12020-012-9849-y

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