Abstract
Thyroid hormones increase energy expenditure and bone turnover in vivo. To study whether 3,3′,5-triiodo-l-thyronine (T3) stimulates the uptake of glucose in osteoblastic cells, PyMS (a cell line derived from rat bone) cells were kept in serum-free culture medium and treated with T3. We measured [1-14C]-2-deoxy-d-glucose (2DG) uptake and looked for expression of the high-affinity glucose transporters GLUT1 and GLUT3 by northern and western analysis. T3 did not influence the cell number but slightly (1.3-fold) increased the protein content of the cell cultures. 2DG uptake was low in serum-deprived cell cultures and was increased by T3 (up to 2.5-fold at 1 nmol l−1 after 4 days) in a dose- and time-dependent manner. Triiodothyronine at 1 nmol l−1 increased GLUT1 and GLUT3 abundance in membranes. Therefore, increased glucose uptake induced by T3 in osteoblasts may be mediated by the known high-affinity glucose transporters GLUT1 and GLUT3.
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Acknowledgments
We thank Martina Gosteli for advice with the preparation of the GLUT cDNA probes, Oliver Tschopp for help with the statistical analysis, and Michèle Rothfuchs for help with the preparation of the manuscript. This work has been supported by the Swiss National Science Foundation (grant 32-46808.96).
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The authors declare that there is no conflict of interest that could be perceived as prejudicing the impartiality of the research reported.
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Zoidis, E., Ghirlanda-Keller, C. & Schmid, C. Triiodothyronine stimulates glucose transport in bone cells. Endocrine 41, 501–511 (2012). https://doi.org/10.1007/s12020-012-9594-2
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DOI: https://doi.org/10.1007/s12020-012-9594-2