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Effects of estrogen receptor α and β gene deletion on estrogenic induction of progesterone receptors in the locus coeruleus in female mice

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Abstract

Locus coeruleus (LC) is involved in the LHRH regulation by gonadal steroids. We investigated the expression of progesterone and estrogen receptors (PR; ER) in LC neurons of ERα (αERKO) or ERβ (βERKO) knockout mice, and their wild-type (αWT and βWT). Immunocytochemical studies showed that LC expresses PR and both ERs, although ERβ was more abundant. Estradiol benzoate (EB) decreased ERα-positive cells in WT and βERKO mice, and progesterone caused a further reduction, whereas none of the steroids influenced ERβ expression. ERβ deletion increased ERα while ERα deletion did not alter ERβ expression. In both WT mice, EB increased PR expression, which was diminished by progesterone. These steroid effects were also observed in αERKO animals but to a lesser extent, suggesting that ERα is partially responsible for the estrogenic induction of PR in LC. Steroid effects on PR in βERKO mice were similar to those in the αERKO but to a lesser extent, probably because PR expression was already high in the oil-treated group. This expression seems to be specific of LC neurons, since it was not observed in other areas studied, the preoptic area and ventromedial nucleus of hypothalamus. These findings show that LC in mice expresses αER, βER, and PR, and that a balance between them may be critical for the physiological control of reproductive function.

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Acknowledgments

The authors gratefully acknowledge Dr. Celso R. Franci, for his generous support for the steroid radioimmunoassay and Ms. Sonia M. Zanon Batista for her technical assistance. This study was supported by CAPES and FAPESP (CVH) and NIMH 62147 (SO).

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Authors and Affiliations

  1. Department of Physiology, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil

    Cleyde Helena

  2. Laboratory of Neurobiology and Behavior, The Rockefeller University, New York, NY, USA

    Cleyde Helena, Donald Pfaff & Sonoko Ogawa

  3. Department of Biosciences and Nutrition, Karolinska Institute, Huddinge, S-141 86, Sweden

    Jan-Åke Gustafsson

  4. Center for Nuclear Receptors and Cell Signaling, Department of Biology and Biochemistry, University of Houston, Houston, TX, 7720, USA

    Jan-Åke Gustafsson

  5. Laboratory of Reproductive and Developmental Toxicology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC, 27709, USA

    Kenneth Korach

  6. Laboratory of Neuroendocrinology of Reproduction, School of Dentistry of Ribeirão Preto, University of São Paulo, Av. Do Café s/n, Ribeirão Preto, SP, 14040-904, Brazil

    Janete A. Anselmo-Franci

  7. Department of Physiology, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil

    Janete A. Anselmo-Franci

  8. Laboratory of Behavioral Neuroendocrinology, Kansei, Behavioral and Brain Sciences Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, 305-8577, Ibaraki, Japan

    Sonoko Ogawa

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  1. Cleyde Helena
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Correspondence to Janete A. Anselmo-Franci.

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Helena, C., Gustafsson, JÅ., Korach, K. et al. Effects of estrogen receptor α and β gene deletion on estrogenic induction of progesterone receptors in the locus coeruleus in female mice. Endocr 36, 169–177 (2009). https://doi.org/10.1007/s12020-009-9207-x

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  • DOI: https://doi.org/10.1007/s12020-009-9207-x

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