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Interleukin-6 stimulates lipolysis in porcine adipocytes

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Abstract

Interleukin (IL)-6 stimulates lipolysis in human and rodents adipocytes. However, the mechanism regulating this process is little known. In this study, we demonstrated that IL-6 increased lipolysis in differentiated porcine adipocytes by activation of extracellular signal-related kinase (ERK), which was inhibited by specific ERK inhibitor PD98059. IL-6 treatment did not elevate intracellular cAMP and specific PKA inhibitor H89 did not affect IL-6-induced lipolysis, which suggested that protein kinase A (PKA) pathway was not involved in IL-6-induced lipolysis. Also, the expressions of perilipin A and PPARγ2 were significantly reduced in response to IL-6 treatment, but the expressions of peroxisome proliferators-activated receptor gamma coactivator-1 alpha (PGC-1α), carnitinepalmitoyl-transferase-1 (CPT-1), and uncoupling protein 2 (UCP2) were significantly elevated. In conclusion, these results suggested that chronic high dose of IL-6 directly stimulated lipolysis in porcine adipocytes through activation of ERK, subsequently repressing perilipin A and promoting PGC-1α expression.

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Abbreviations

IL-6:

Interleukin-6

PPARγ2:

Peroxisome proliferators-activated receptor-gamma 2

HSL:

Hormone-sensitive lipase

ATGL:

Adipose triglyceride lipase

PGC-1α:

Peroxisome proliferators-activated receptor gamma coactivator-1 alpha

CPT-1:

Carnitinepalmitoyl-transferase-1

UCP2:

Uncoupling protein 2

FFA:

Free fatty acids

Semi-q RT-PCR:

Semi-quantitative reverse transcription polymerase chain reaction

ERK:

Extracellular signal-related kinase

PKA:

Protein kinase A

cAMP:

Cyclic adenosine monophosphate

MAPK:

Mitogen-activated protein kinase

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Acknowledgments

We thank Dr. Bin Wu (Arizona Center for Reproduction, USA) for his helpful suggestions and correction of the English manuscript. This research was sponsored by The National High Technology Research and Development Program (“863”Program) of China (Grant NO. 2006AA10Z138) and National Basic Research Program (“973”Program) of China (Grant NO. 2004CB117506).

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Authors and Affiliations

  1. Laboratory of Animal Fat Deposition and Muscle Development, College of Animal Science and Technology, Northwest A&F University, 22 Xinong Road, Yangling, Shaanxi Province, 712100, People’s Republic of China

    Yongqing Yang, Dapeng Ju, Mingtao Zhang & Gongshe Yang

  2. College of Life Science, Shanxi Normal University, The First Gongyuan Street, Linfen, Shanxi Province, 041000, People’s Republic of China

    Yongqing Yang

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  1. Yongqing Yang
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Correspondence to Gongshe Yang.

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Yang, Y., Ju, D., Zhang, M. et al. Interleukin-6 stimulates lipolysis in porcine adipocytes. Endocr 33, 261–269 (2008). https://doi.org/10.1007/s12020-008-9085-7

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