Abstract
In this study, using the H295R cell line as a model system, we investigated the role of prolactin (PRL) and steroid hormones in the growth regulation and cortisol release of adrenocortical cells. H295R cells were treated with increasing doses (10−13–10−6 M) of PRL, adrenocorticotropic hormone (ACTH), 17β-estradiol (E2), progesterone (P4), testosterone (T), and dihydrotestosterone (DHT). As expected, ACTH raised cortisol secretion and increased the proliferation rate of cultured cells. Incubation with T, DHT, E2, and P4 for 24 h did not significantly increase cortisol release. Conversely, PRL concentrations of 10−8–10−6 M caused a significant increase in the release of cortisol. Long-term (5 days) stimulation of H295R cells with E2, P4, and PRL was a trigger to increased cell proliferation, while T and DHT did not alter H295R cell proliferation. Taken together, these results indicate that steroid hormones exert differential effects on adrenocortical function. Additionally, the present study demonstrates that PRL had biphasic actions in regulating adrenocortical function. PRL may form a novel regulatory system for steroid hormone secretion and cell proliferation in the adrenal cortex.
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L.B. McDonough, J.E. Ewig, Comp. Biochem. Physiol. A 72, 259–261 (1982)
H.G. Klemcke, W.G. Pond, J.A. Nienaber, Comp. Biochem. Physiol. A 92, 197–206 (1989)
A. Ouhtit, G. Morel, P.A. Kelly, Endocrinology 133, 135–144 (1993)
A. Glasow, M. Breidert, A. Haidan, U. Anderegg, P.A. Kelly, S.R. Bornstein, J. Clin. Endocrinol. Metab. 81, 3103–3111 (1996)
A. Glasow, A. Haidan, J. Gillespie, P.A. Kelly, G.P. Chrousos, S.R. Bornstein, Endocr. Res. 24, 857–862 (1999)
M.J. Lo, M.M. Kau, Y.H. Chen, S.C. Tsai, Y.C. Chiao, J.J. Chen, C. Liaw, C.C. Lu, B.P. Lee, S.C. Chen, V.S. Fang, L.T. Ho, P.S. Wang, Am. J. Physiol. 74, E238–E245 (1998)
L.L. Chang, M.J. Lo, S.F. Kan, W.J. Huang, J.J. Chen, M.M. Kau, J.L. Wang, H. Lin, S.C. Tsai, Y.C. Chiao, J.Y. Yeh, W.S. Wun, P.S. Wang, J. Cell Biochem. 73, 563–572 (1999)
M.M. Kau, M.J. Lo, S.C. Tsai, J.J. Chen, H.F. Pu, E.J. Chien, L.L. Chang, P.S. Wang, J. Cell Biochem. 72, 286–293 (1999)
M.J. Lo, P.S. Wang, J. Cell Biochem. 85, 35–41 (2002)
S.F. Kan, M.M. Kau, L. Low-Tone Ho, P.S. Wang, Eur. J. Pharmacol. 468, 141–149 (2003)
M.J. Lo, M.M. Kau, P.S. Wang, J. Cell Biochem. 97, 351–358 (2006)
S. Jaroenporn, K. Nagaoka, R. Ohta, G. Watanabe, K. Taya, J. Reprod. Dev. 53, 887–893 (2007)
V.S. Fang, S. Refetoff, R.L. Rosenfield, Endocrinology 95, 991–998 (1974)
A. Bartke, M.S. Smith, S.D. Michael, F.G. Peron, S. Dalterio, Endocrinology 100, 182–186 (1977)
A. Bartke, P.C. Doherty, R.W. Steger, W.W. Morgan, A.G. Amador, D.C. Herbert, T.M. Siler-Khodr, M.S. Smith, H.G. Klemcke, W.C. Hymer, Neuroendocrinology 39, 126–135 (1984)
E.J. Silva, L.F. Felicio, A.G. Nasello, M. Zaidan-Dagli, J.A. Anselmo-Franci, Braz. J. Med. Biol. Res. 37, 193–199 (2004)
C. Bole-Feysot, V. Goffin, M. Edery, N. Binart, P.A. Kelly, Endocr. Rev. 19, 225–268 (1998)
A. Crépin, G. Bidaux, F. Vanden-Abeele, E. Dewailly, V. Goffin, N. Prevarskaya, C. Slomianny, Biochem. J. 401, 49–55 (2007)
F. Van Coppenolle, R. Skryma, H. Ouadid-Ahidouch, C. Slomianny, M. Roudbaraki, P. Delcourt, E. Dewailly, S. Humez, A. Crépin, I. Gourdou, J. Djiane, J.L. Bonnal, B. Mauroy, N. Prevarskaya, Biochem. J. 377, 569–578 (2004)
S. Jaroenporn, K. Nagaoka, R. Ohta, M. Shirota, G. Watanabe, K. Taya, Stress (2008 in press)
L. Barzon, N. Sonino, F. Fallo, G. Palu, M. Boscaro, Eur. J. Endocrinol. 149, 273–285 (2003)
A.W. Hsing, J.M. Nam, H.T. Co Chien, J.K. McLaughlin, J.F Fraumeni Jr., Int. J. Cancer 65, 432–436 (1996)
D. Montanaro, M. Maggiolini, A.G. Recchia, R. Sirianni, S. Aquila, L. Barzon, F. Fallo, S. Andò, V. Pezzi, J. Mol. Endocrinol. 35, 245–256 (2005)
R. Rossi, M.C. Zatelli, A. Valentini, P. Cavazzini, F. Fallo, L. del Senno, E.C. degli Uberti, J. Endocrinol. 159, 373–380 (1998)
M.C. Zatelli, R. Rossi, E.C. degli Uberti, J. Clin. Endocrinol. Metab. 85, 847–852 (2000)
D. Somjen, N. Stern, E. Knoll, O. Sharon, B. Gayer, T. Kulik, F. Kohen, J. Endocrinol. 179, 395–403 (2003)
L. Barzon, G. Masi, M. Pacenti, M. Trevisan, F. Fallo, A. Remo, G. Martignoni, D. Montanaro, V. Pezzi, G. Palù, Virchows Arch. 452, 181–191 (2008)
K. Arai, G. Watanabe, M. Fujimoto, S. Nagata, Y. Takemura, K. Taya, S. Sasamoto, J. Reprod. Dev. 41, j15–j20 (1995)
Acknowledgments
We are grateful to Dr. A.F. Parlow and the Rat Pituitary Hormone Distribution Program (NIDDK, NIH, Torrance, CA, USA) for providing recombinant human PRL (AFP 795) for in vitro study and to the Institute for Molecular and Cellular Regulation, University of Gunma, Gunma, Japan, for providing antisera to cortisol. This study was supported in part by a Grant-in-Aid for Scientific Research (B-18310044, The Japan Thailand Joint Research) from the Ministry of Education, Culture, Sport, Science and Technology of Japan, and the Japan Society for the Promotion of Sciences (JSPS).
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Jaroenporn, S., Furuta, C., Nagaoka, K. et al. Comparative effects of prolactin versus ACTH, estradiol, progesterone, testosterone, and dihydrotestosterone on cortisol release and proliferation of the adrenocortical carcinoma cell line H295R. Endocr 33, 205–209 (2008). https://doi.org/10.1007/s12020-008-9075-9
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DOI: https://doi.org/10.1007/s12020-008-9075-9