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ID2 promotes the expansion and survival of growth-arrested pancreatic beta cells

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Abstract

Inhibitors of DNA binding proteins (Ids) are implicated in the control of proliferation and differentiation. Herein, we tested the hypothesis that Id2 could stimulate proliferation and survival in differentiated pancreatic beta cells. We showed that Id2-enhanced proliferation of a growth-arrested pancreatic beta cell line (BTC-tet). This was mediated by the Rb pathway, as shown by an E2F1-driven reporter assay and Western immunoblot of phosphorylated Rb protein. Id2 also induced expression of Bcl-2, accompanied by a significant reduction of critical mediators of cytokine stimulation, including p38 MAPK and NFκB, as well as apoptosis markers, caspase-3 and Annexin-V. Overall, our data suggest that Id2 enhances proliferation and survival of growth-arrested BTC-tet cells.

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Acknowledgments

This study was generously funded by the Larry L. Hillblom Foundation to HH and NIH grant DK060746 to NS (Scripps manuscript number 18782). We thank the generosity of Jayshree Sarnater and John Kessler for the Id2 construct, Joseph Nevins for the E2F1-luciferase reporter, Shimon Efrat for the BTC-tet cells, and Sandrine Arnaud-Dabernat for the BTC-tet cells expressing the E2F1-luciferase reporter. We also thank members of the Sarvetnick lab for critical reading of the manuscript.

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  1. Department of Immunology, The Scripps Research Institute, 10550 N. Torrey Pines Rd., IMM-23, La Jolla, CA, 92037, USA

    Hong Hua & Nora Sarvetnick

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  1. Hong Hua
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  2. Nora Sarvetnick
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Correspondence to Nora Sarvetnick.

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Hua, H., Sarvetnick, N. ID2 promotes the expansion and survival of growth-arrested pancreatic beta cells. Endocr 32, 329–337 (2007). https://doi.org/10.1007/s12020-008-9039-0

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  • DOI: https://doi.org/10.1007/s12020-008-9039-0

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