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The Identification of Neuropeptide Y Receptor Subtype Involved in Phenylpropanolamine-induced Increase in Oxidative Stress and Appetite Suppression

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Abstract

Hypothalamic neuropeptide Y (NPY) and superoxide dismutase (SOD) have been reported to participate in the regulation of appetite-suppressing effect of phenylpropanolamine (PPA), a sympathomimetic agent. This study explored whether Y1 receptor (Y1R) and/or Y5 receptor (Y5R) was involved in this regulation. Wistar rats were treated with PPA for 24 h. Changes in food intake and hypothalamic NPY, Y1R, Y5R, and SOD contents were assessed and compared. Results showed that food intake and NPY contents were decreased following PPA treatment, while Y1R and SOD contents were increased and Y5R contents remained unchanged. Moreover, although Y1R or Y5R knockdown by themselves could modify the food intake, Y1R but not Y5R knockdown could modify PPA-induced anorexia as well as NPY and SOD contents. In addition, selective inhibition of Y1R but not Y5R could modulate PPA-induced anorexia. It is suggested that Y1R but not Y5R participates in the anorectic response of PPA via the modulation of NPY and SOD. Results provide molecular mechanism of NPY-mediated PPA anorexia and may aid the understanding of the toxicology of PPA.

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Acknowledgments

This study was supported by a grant from the National Science Council (NSC- 101-2320-B-040-002-MY3).

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There is no conflict of interest.

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Authors and Affiliations

  1. Institute of Biochemistry and Biotechnology, Chung Shan Medical University and Chung Shan Medical University Hospital, Taichung City, 40201, Taiwan, ROC

    Yih-Shou Hsieh & Pei-Ni Chen

  2. National Taichung Second Senior High School, Taichung City, 404, Taiwan, ROC

    Meng-Hsien Kuo

  3. Department of Physiology, Chung Shan Medical University and Chung Shan Medical University Hospital, Taichung City, 40201, Taiwan, ROC

    Dong-Yih Kuo

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  1. Yih-Shou Hsieh
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  2. Meng-Hsien Kuo
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Correspondence to Dong-Yih Kuo.

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Dr. Yih-Shou Hsieh and Mr. Meng-Hsien Kuo contributed equally to this research.

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Hsieh, YS., Kuo, MH., Chen, PN. et al. The Identification of Neuropeptide Y Receptor Subtype Involved in Phenylpropanolamine-induced Increase in Oxidative Stress and Appetite Suppression. Neuromol Med 15, 159–168 (2013). https://doi.org/10.1007/s12017-012-8206-x

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