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Molecular Pathways Governing Development of Vascular Endothelial Cells from ES/iPS Cells

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Abstract

Assembly of complex vascular networks occurs in numerous biological systems through morphogenetic processes such as vasculogenesis, angiogenesis and vascular remodeling. Pluripotent stem cells such as embryonic stem (ES) and induced pluripotent stem (iPS) cells can differentiate into any cell type, including endothelial cells (ECs), and have been extensively used as in vitro models to analyze molecular mechanisms underlying EC generation and differentiation. The emergence of these promising new approaches suggests that ECs could be used in clinical therapy. Much evidence suggests that ES/iPS cell differentiation into ECs in vitro mimics the in vivo vascular morphogenic process. Through sequential steps of maturation, ECs derived from ES/iPS cells can be further differentiated into arterial, venous, capillary and lymphatic ECs, as well as smooth muscle cells. Here, we review EC development from ES/iPS cells with special attention to molecular pathways functioning in EC specification.

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Acknowledgments

We thank Drs. Kenzaburo Tani and Koichi Akashi and Sugiyama lab members for discussion, Dr. Elise Lamar for editing the manuscript, and the Ministry of Education, Culture, Sports, Science and Technology, the Ministry of Health, Labor and Welfare, the Japan Society for the Promotion of Science, the Ichiro Kanehara Foundation for the Promotion of Medical Sciences and Medical care, and Institute of Molecular Embryology and Genetics for grant support. Keai Sinn Tan is a recipient of scholarship from The Tokyo Biochemical Research Foundation, Japan and MyPhD scholarship from Ministry of Higher Education (MOHE), Malaysia.

Conflict of Interest Statement

The authors indicate no potential conflicts of interest.

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Authors and Affiliations

  1. ARO Center for Translational and Clinical Research, Kyushu University Hospital, Station for Collaborative Research 1, 4 F, 3-1-1 Maidashi, Higashi-Ku, Fukuoka, 812-8582, Japan

    Keai Sinn Tan, Yoichi Nakanishi & Daisuke Sugiyama

  2. Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor Darul Ehsan, Malaysia

    Keai Sinn Tan & Mei I Lai

  3. Department of Cell Differentiation, Institute of Molecular Embryology and Genetics, Kumamoto University, 2-2-1 Honjo, Kumamoto, 860-0811, Japan

    Kiyomi Tamura & Minetaro Ogawa

  4. Medical Genetics Laboratory, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor Darul Ehsan, Malaysia

    Abhimanyu Veerakumarasivam

  5. UPM-MAKNA Cancer Research Laboratory, Institute Bioscience, Universiti Putra Malaysia, 43400, Serdang, Selangor Darul Ehsan, Malaysia

    Abhimanyu Veerakumarasivam

  6. Perdana University Graduate School of Medicine, Perdana University, 43400, Serdang, Selangor Darul Ehsan, Malaysia

    Abhimanyu Veerakumarasivam

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  1. Keai Sinn Tan
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  2. Kiyomi Tamura
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  3. Mei I Lai
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  4. Abhimanyu Veerakumarasivam
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  5. Yoichi Nakanishi
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  6. Minetaro Ogawa
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  7. Daisuke Sugiyama
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Correspondence to Daisuke Sugiyama.

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Tan, K.S., Tamura, K., Lai, M.I. et al. Molecular Pathways Governing Development of Vascular Endothelial Cells from ES/iPS Cells. Stem Cell Rev and Rep 9, 586–598 (2013). https://doi.org/10.1007/s12015-013-9450-7

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  • DOI: https://doi.org/10.1007/s12015-013-9450-7

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