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Cell Therapy with Bone Marrow Mononuclear Cells in Elastase-Induced Pulmonary Emphysema

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Abstract

Emphysema is characterized by destruction of alveolar walls with loss of gas exchange surface and consequent progressive dyspnea. This study aimed to evaluate the efficiency of cell therapy with bone marrow mononuclear cells (BMMC) in an animal model of elastase-induced pulmonary emphysema. Emphysema was induced in C57Bl/J6 female mice by intranasal instillation of elastase. After 21 days, the mice received bone marrow mononuclear cells from EGFP male mice with C57Bl/J6 background. The groups were assessed by comparison and statistically significant differences (p < 0.05) were observed among the groups treated with BMMC and evaluated after 7, 14 and 21 days. Analysis of the mean linear intercept (Lm) values for the different groups allowed to observe that the group treated with BMMC and evaluated after 21 days showed the most significant result. The group that received no treatment showed a statistically significant difference when compared to other groups, except the group treated and evaluated after 21 days, evidencing the efficacy of cell therapy with BMMC in pulmonary emphysema.

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Authors and Affiliations

  1. Inter-units Program of Post-Graduation in Biotechnology, University of São Paulo – USP – Butantan Inst. – IPT, São Paulo, SP, Brazil

    Nathalia Longhini-dos-Santos, Valter Abraão Barbosa-de-Oliveira, Rodrigo Heras Kozma, Carolina Arruda de Faria & Talita Stessuk

  2. Department of Biological Sciences, University of the State of São Paulo – UNESP, Assis, SP, Brazil

    Fernando Frei & João Tadeu Ribeiro-Paes

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  1. Nathalia Longhini-dos-Santos
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  2. Valter Abraão Barbosa-de-Oliveira
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Correspondence to João Tadeu Ribeiro-Paes.

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Longhini-dos-Santos, N., Barbosa-de-Oliveira, V.A., Kozma, R.H. et al. Cell Therapy with Bone Marrow Mononuclear Cells in Elastase-Induced Pulmonary Emphysema. Stem Cell Rev and Rep 9, 210–218 (2013). https://doi.org/10.1007/s12015-012-9419-y

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