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Cancer-Initiating Enriched Cell Lines from Human Glioblastoma: Preparing for Drug Discovery Assays

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Abstract

Glioblastoma multiforme (GBM) is the most lethal type of brain tumour in the adult humans. The cancer-Initiatingcell (CIC) hypothesis supports the notion that failures in current approaches to GBM treatment might be attributed to the survival of the CIC subpopulation. Recent evidence shows the idea that using CIC-enriched cell lines derived from human GBM as new targets for drug discovery programs, may improve the chance of successfully translating the basic research findingsinto clinical trials. Although this approach appears promising, many important biological and technical issues (characterization of functional CIC markers, inter- and intra-tumoral CIC heterogeneity, and isolation and maintenance inconsistency) need to be resolved.

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Acknowledgment

We are especially grateful to Dr. Escobedo-Lucea for her technical advice. Miriam Romaguera-Ros is recipient of a Predoctoral Fellowship from The FIS program (FI05/0087), Ministerio de Sanidad, Spain. Jorge Oliver De La Cruz and Arantxa Pérez García are recipients of a Predoctoral Fellowship from The FPU program (AP2008/02823 and AP2009/1317), Ministerio de Educacion y Ciencia, Spain. This work was supported in part by grants from Generalitat Valenciana (GV/2009/68) (AAS), The Gent x Gent Foundation (AAS) and Fondo de Investigaciones Sanitarias (FIS) del instituto de Salud Carlos III (PI10/01069)(AAS).

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All authors have read and have approved of the manuscripts in their final form. Al authors declare that there is not conflict of interest with any material included in the manuscript.

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Authors and Affiliations

  1. Department of Cell Morphology, Centro de Investigación Príncipe Felipe and RETICS-CIBERNED, AVDA. Autopista del Saler, 16, 46012, Valencia, Spain

    Miriam Romaguera-Ros, Jorge Oliver-De La Cruz, Josefa Carrión-Navarro, Arantxa Pérez-García & Jose Manuel García-Verdugo

  2. Department of Neurosurgery, Hospital la Fe, Valencia, Spain

    María Peris-Celda

  3. Universidad de Valencia, Valencia, Spain

    Jose Manuel García-Verdugo

  4. New York Brain Tumor Research Center, Weill Cornell Medical College of Cornell University, New York, NY, USA

    Angel Ayuso-Sacido

  5. Deparment of Cell Morphology, Centro de Investigación Príncipe Felipe, REIG and CIBERNED, AVDA Autopista del Saler 16, 46012, Valencia, Spain

    Angel Ayuso-Sacido

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  1. Miriam Romaguera-Ros
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  2. María Peris-Celda
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  3. Jorge Oliver-De La Cruz
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Correspondence to Jose Manuel García-Verdugo or Angel Ayuso-Sacido.

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Miriam Romaguera-Ros and María Peris-Celda have equal contribution.

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Romaguera-Ros, M., Peris-Celda, M., Oliver-De La Cruz, J. et al. Cancer-Initiating Enriched Cell Lines from Human Glioblastoma: Preparing for Drug Discovery Assays. Stem Cell Rev and Rep 8, 288–298 (2012). https://doi.org/10.1007/s12015-011-9283-1

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