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Nuclear Proteome Analysis of Monkey Embryonic Stem Cells During Differentiation

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An Erratum to this article was published on 27 February 2010

An Erratum to this article was published on 27 February 2010

Abstract

The nuclear proteome enables, manages, and regulates the genome by the collective actions and interactions of proteins found in the nucleus. We applied a proteomic approach to analyze a nuclear proteome during embryonic stem cell (ESC) proliferation, and 3 and 9 days after initiation of differentiation. The nuclei were isolated from cells and their proteins were separated using 2-DE. Out of about 560 protein spots reproducible detected on any give gel, 49 differentially expressed proteins were identified by Matrix Assisted Laser Desorption Ionization-Time of Flight (MALDI TOF/TOF) mass spectrometry. Of them, several nuclear located proteins involved in chromatin remodeling, transcription regulation, apoptosis, cell proliferation, and differentiation were identified including CTBP1, MM-1, RUVBL1, HCC-1, SGTA, SUMO2, and Galectin-1. Functional interaction analysis of differentially expressed proteins revealed that most of nuclear proteins had a direct interaction with c-Myc and p53.

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Acknowledgement

This study was funded by a grant provided from Royan Institute.

Disclosures

The authors indicate no potential conflicts of interest.

Author information

Authors and Affiliations

  1. Department of Molecular Systems Biology, Royan Institute for Stem Cell Biology and Technology, ACECR, P.O. Box: 19395-4644, Karaj, Iran

    Davood Nasrabadi, Ali Fathi & Ghasem Hosseini Salekdeh

  2. Department of Stem Cells and Developmental Biology, Royan Institute for Stem Cell Biology and Technology, ACECR, P.O. Box: 19395-4644, Tehran, Iran

    Mehran Rezaei Larijani & Hossein Baharvand

  3. Department of Genetics, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran

    Hamid Gourabi

  4. Department of Reproductive Imaging, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran

    Ahmad V. Dizaj

  5. Department of Developmental Biology, University of Science and Culture, ACECR, Tehran, Iran

    Hossein Baharvand

  6. Department of Systems Biology, Agricultural Biotechnology Research Institute of Iran, Karaj, Iran

    Ghasem Hosseini Salekdeh

Authors
  1. Davood Nasrabadi
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  2. Mehran Rezaei Larijani
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  3. Ali Fathi
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  4. Hamid Gourabi
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  6. Hossein Baharvand
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  7. Ghasem Hosseini Salekdeh
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Corresponding authors

Correspondence to Hossein Baharvand or Ghasem Hosseini Salekdeh.

Additional information

An erratum to this article can be found at http://dx.doi.org/10.1007/s12015-010-9127-4

Electronic Supplemantary Material

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Fig. S1

Morphological and flow cytometry analyses of undifferentiated and differentiated monkey ESCs. a Phase contrast photomicrographs of a colony of monkey ESCs grown on feeder cells. b High magnification of ESCs. Each cell displays a compact morphology and a high nucleus-cytoplasmic ratio containing prominent nucleoli, typical of undifferentiated ESC. c The ESC line had a normal karyotype (42 XY). Representative flow cytometric analysis of key ESC markers, including Oct-4 d, SSEA-4 e, Tra-1-60 f, and Tra-1-81 g. The cells expressing markers compared with isotype control (white peaks) were termed as the marker-positive population. Percentages of double positive for SSEA-4/TRA-1-60 h and SSEA-4/TRA-1-81 i are indicated in the dot plots. The percentages of undifferentiated and differentiated ESCs are presented in l. The differentiating EBs were cultured in suspension for 9 days (Fig. 1 j = day 3, k = day 9) (DOC 188 kb)

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Nasrabadi, D., Larijani, M.R., Fathi, A. et al. Nuclear Proteome Analysis of Monkey Embryonic Stem Cells During Differentiation. Stem Cell Rev and Rep 6, 50–61 (2010). https://doi.org/10.1007/s12015-009-9109-6

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