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Isolation of Mesenchymal Stem Cells from Human Placental Decidua Basalis and Resistance to Hypoxia and Serum Deprivation

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Abstract

Mesenchymal stem cells (MSCs) are the most promising seed cells for cell therapy and tissue engineering, which can be isolated from various sources of human adult tissues such as bone marrow and adipose tissue. However, cells from these tissues must be obtained through invasive procedures and sometimes the individual difference is hard to control. Hence, the search continues for an ethically conducive, easily accessible and controllable source of stem cells. We herein report the isolation of a population of stem cells from the human placental decidua basalis (termed as PDB-MSCs), a maternal portion of placenta. PDB-MSCs were further shown to express markers common to MSCs and positive for SSEA-1, SSEA-3, SSEA-4, TRA-1-60, TRA-1-81 and Oct-4. In order to facilitate the further utility in ischemic diseases, we tested the apoptosis of PDB-MSCs in hypoxia and serum deprivation, two components of ischemia in vivo. Taken together, our findings indicate that PDB-MSCs are resistant to hypoxia and serum deprivation, which may relate to Bcl-2.

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Acknowledgements

This work was supported by National High Technology Research and Development Program (“863”) of China (2007AA021900) and the Key Project of Science and Technology Bureau of Sichuan province (No.07SG111-004 and No.2008SZ0035). The authors are indebted to Dr. Guang-Qian Zhou for expert review of the manuscript.

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Authors and Affiliations

  1. Division of Stem cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, People’s Republic of China

    Yong-Can Huang, Zhi-Ming Yang, Xiao-He Chen, Mei-Yun Tan, Jia Wang, Xiu-Qun Li, Hui-Qi Xie & Li Deng

  2. West China School of Pharmacy, Sichuan University, Chengdu, 610041, People’s Republic of China

    Yong-Can Huang & Jia Wang

  3. Division of Stem cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, West China School of Medicine, Sichuan University, No.1 Ke-Yuan-Si-Lu, Gao-Peng-Da-Dao, Chengdu, Sichuan, 610041, People’s Republic of China

    Li Deng

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  1. Yong-Can Huang
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Huang, YC., Yang, ZM., Chen, XH. et al. Isolation of Mesenchymal Stem Cells from Human Placental Decidua Basalis and Resistance to Hypoxia and Serum Deprivation. Stem Cell Rev and Rep 5, 247–255 (2009). https://doi.org/10.1007/s12015-009-9069-x

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