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Prospective Isolation and Functional Analysis of Stem and Differentiated Cells from the Mouse Mammary Gland

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Abstract

Prospective isolation and in vitro and in vivo analysis of primary mouse mammary epithelial cells has been used to separate cell subpopulations and identify stem, progenitor and differentiated cell compartments. Progress has been made from cell separation strategies based on a single marker of the luminal epithelial or myoepithelial compartments to use of markers that allow simultaneous isolation of non-epithelial, basal/myoepithelial and luminal epithelial cells. Transplant analysis has shown that mammary stem cells are found in the basal/myoepithelial compartment, whereas in vitro colony progenitors are found in the luminal compartment. A basal population enriched for stem cell activity can be purified from the myoepithelial cells and the most recent data shows that the luminal population can now be prospectively split into estrogen receptor positive and estrogen receptor negative cells. Future work aims to molecularly characterise these populations to identify new drug targets, which can be used to specifically kill breast cancer stem cells.

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Acknowledgements

The authors would like to thank all those who contributed to or advised on the studies described above, in particular Katherine Sleeman, Azra Alvi, Mike O’Hare, Trevor Dale, Tariq Enver, Clare Isacke and Alan Ashworth.

Work in the laboratory is supported by Breakthrough Breast Cancer.

Author information

Authors and Affiliations

  1. The Breakthrough Breast Cancer Research Centre, Institute of Cancer Research, 237 Fulham Road, London, SW3 6JB, UK

    Joseph Regan & Matthew Smalley

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  1. Joseph Regan
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  2. Matthew Smalley
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Correspondence to Matthew Smalley.

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Regan, J., Smalley, M. Prospective Isolation and Functional Analysis of Stem and Differentiated Cells from the Mouse Mammary Gland. Stem Cell Rev 3, 124–136 (2007). https://doi.org/10.1007/s12015-007-0017-3

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  • DOI: https://doi.org/10.1007/s12015-007-0017-3

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