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TNF-α, IL-6, and Leptin Increase the Expression of miR-378, an Adipogenesis-Related microRNA in Human Adipocytes

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Abstract

Obesity has become a global public health problem associated with complications including type 2 diabetes, cardiovascular disease, and several cancers. Adipocyte differentiation (adipogenesis) plays an important role in obesity and energy homeostasis. Adipose tissue secretes multiple cytokines and adipokines which can cause the complications of obesity, especially insulin resistance. TNF-α, IL-6, leptin, and resistin have been identified as the main regulators of obesity and insulin activity. miR-378 is highly induced during adipogenesis and has been reported to be positively regulated in adipogenesis. In the current study, matured human adipocytes were treated with TNF-α, IL-6, leptin, or resistin on the 15th day after the induction of human pre-adipocyte differentiation. We demonstrated that TNF-α, IL-6, and leptin upregulated miR-378 expression indicating that miR-378 probably is a novel mediator in the development of insulin resistance related to obesity.

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Acknowledgments

This work was supported by Grants from the National Key Basic Research Program of China (2013CB530604), the National Natural Science Foundation of China (81270928), the Program for Innovative Research Teams of Jiangsu Province (LJ201108), the Natural Science Foundation of Jiangsu Province (BK2011107), and Nanjing Technological Development Program (201104013).

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Authors and Affiliations

  1. Department of Pediatrics, Affiliated Hospital of Nantong University, Nantong, 226000, People’s Republic of China

    Lu-lian Xu & Mei-yu Xu

  2. Nanjing Maternal and Child Health Hospital Affiliated to Nanjing Medical University, Nanjing, 210029, People’s Republic of China

    Chun-mei Shi, Ling Chen, Xi-rong Guo & Chen-bo Ji

  3. Institute of Pediatrics, Nanjing Medical University, Nanjing, 210029, People’s Republic of China

    Chun-mei Shi, Guang-feng Xu, Ling Chen, Ling-ling Zhu, Lu Zhu, Xi-rong Guo & Chen-bo Ji

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  1. Lu-lian Xu
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Correspondence to Mei-yu Xu or Chen-bo Ji.

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Xu, Ll., Shi, Cm., Xu, Gf. et al. TNF-α, IL-6, and Leptin Increase the Expression of miR-378, an Adipogenesis-Related microRNA in Human Adipocytes. Cell Biochem Biophys 70, 771–776 (2014). https://doi.org/10.1007/s12013-014-9980-x

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  • DOI: https://doi.org/10.1007/s12013-014-9980-x

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