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Effect of Oxidative Stress on the Expression of Thin Filament-Associated Proteins in Gastric Smooth Muscle Cells

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Abstract

Thin filament-associated proteins such as calponin, caldesmon, and smoothelin are believed to regulate acto-myosin interaction and thus, muscle contraction. Oxidative stress has been found to affect the normal contractile behavior of smooth muscle and is involved in the pathogenesis of a number of human diseases such as diabetes mellitus, hypertension, and atherosclerosis. However, very little is known about the effect of oxidative stress on the expression of smooth muscle contractile proteins. The aim of the current study is to investigate the effect of oxidative stress on the expression of thin filament-associated proteins in rat gastric smooth muscle. Single smooth muscle cells of the stomach obtained from Sprague–Dawley rats were used. Muscle cells were treated with hydrogen peroxide (H2O2) (500 μM) for 30 min or the peroxynitrite donor 3-morpholinosydnonimine (SIN-1) (1 mM) for 90 min to induce oxidative stress. Calponin, caldesmon, and smoothelin expressions were measured via specifically designed enzyme-linked immunosorbent assay. We found that exposure to exogenous H2O2 or incubation of dispersed gastric muscle cells with SIN-1 significantly increased the expression of calponin, caldesmon, and smoothelin proteins. In conclusion: oxidative stress increases the expression of thin filament-associated proteins in gastric smooth muscle, suggesting an important role in gastrointestinal motility disorders associated with oxidative stress.

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Acknowledgments

This work was supported by Jordan University of Science & Technology, Irbid, Jordan (Grant Number 93/2013).

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Physiology and Biochemistry, Faculty of Medicine, Jordan University of Science and Technology, Irbid, 22110, Jordan

    Othman Abdullah Al-Shboul, Ayman Mustafa, Mukhallad Mohammad, Mustafa Al-Shehabat, Asmaa Yousef & Farah Al-hashimi

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  1. Othman Abdullah Al-Shboul
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  2. Ayman Mustafa
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Correspondence to Othman Abdullah Al-Shboul.

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Al-Shboul, O.A., Mustafa, A., Mohammad, M. et al. Effect of Oxidative Stress on the Expression of Thin Filament-Associated Proteins in Gastric Smooth Muscle Cells. Cell Biochem Biophys 70, 225–231 (2014). https://doi.org/10.1007/s12013-014-9886-7

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