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Biological Evaluation of a New Synthesized Pt(II) Complex by Cytotoxic and Spectroscopic Studies

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Abstract

In this study, the interaction of β-casein (β-CN) with a new synthesized Pt(II) complex (bipyridin morpholin dithiocarbamate Pt(II) nitrate), as an anticancer compound, was studied. This study was carried by fluorescence and circular dichroism (CD) measurements at 25 and 37° C. Also, cytotoxicity and apoptotic activity of the complex were studied against cancer model cell line of K562 using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Fluorescence data revealed that the intrinsic fluorescence of β-CN was quenched by the addition of Pt(II) complex through dynamic quenching mechanism. The CD spectra indicated that the binding of Pt(II) complex to β-CN causes a slight conformational change in the secondary structure of protein. Also, MTT assay represented growth inhibitory effect of the complex toward the cancer cell line. From above results, it can be concluded that β-CN can bind to the Pt complex and transfer this new anticancer drug. It may be suggested that the antitumor activity of this complex against K562 cell reveals typical morphology features of apoptotic death.

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Abbreviations

β-CN:

β-Casein

Pt(II) complex:

Bipyridin morpholin dithiocarbamate Pt(II) nitrate

CD:

Circular dichroism

ΔH°:

Enthalpy

ΔS°:

Entropy

ΔG°:

Gibbs free energy

MTT:

3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide

DAPI:

4,6-Diamidino-2-phenylindole

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Acknowledgments

The financial support of Research Council of Kharazmi University is highly appreciated.

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Authors and Affiliations

  1. Department of Cell & Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran

    Adeleh Divsalar & Mahdieh Razmi

  2. Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran

    Ali Akbar Saboury

  3. Department of Chemistry, University of Sistan & Baluchestan, Zahedan, Iran

    Hassan Mansouri-Torshizi

  4. Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, New Delhi, India

    Faizan Ahmad

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  1. Adeleh Divsalar
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  2. Mahdieh Razmi
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  5. Faizan Ahmad
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Correspondence to Adeleh Divsalar.

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Divsalar, A., Razmi, M., Saboury, A.A. et al. Biological Evaluation of a New Synthesized Pt(II) Complex by Cytotoxic and Spectroscopic Studies. Cell Biochem Biophys 71, 1415–1424 (2015). https://doi.org/10.1007/s12013-014-0364-z

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