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Expression of Tumor-Associated Macrophage in Progression of Human Glioma

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Abstract

The aim of this study is to investigate the expression of tumor-associated macrophages (TAMs) M1, M2 phenotypic in human glioma tissues, and to explore the clinical significance and prognostic value of TAMs in glioma patients. A total of 50 glioma samples were obtained from patients diagnosed in our hospital from 2007 to 2010. Clinical follow-up was conducted via return visits and telephone interviews after discharge. Progression free survival (PFS) was calculated based on tumor progression by MRI and CT examination from the primary operation. Overall survival (OS) time was calculated from the initial surgical operation date to end date of follow-up or death. Kaplan–Meier methodology was used to evaluate the survival of patients and log-rank test for comparing differences between groups. The expression levels of CD16 and CD206 were investigated in the 4 μm serial paraffin sections by immunohistochemistry. M1-type macrophages filtrated in all the grades of glioma samples, and the lower expression level was associated with high grade glioma. A negative correlation was found between WHO pathological grades and the expression of M1-type macrophages by Spearman correlation analysis. M2-type macrophages filtrated in all the grades of glioma samples with the higher expression level associated with high grade glioma. A positive correlation was found between WHO pathological grades and the expression of M2-type macrophages by Spearman correlation analysis. The PFS and OS among patients with high levels of M1-type macrophages (CD16+++) were significantly higher than those with less expression. The PFS and OS among patients with high levels of M2-type macrophages (CD206+++) were significantly lower than those with low expression. M1-type macrophages may inhibit the tumor growth and improve the therapeutic outcome of glioma patients. M2 ratios are associated with tumor proliferation and poor prognosis. TAMs phenotypes of glioma samples are the potential biomarkers in assessing the degree of malignancy, tumor invasion, and patient prognosis in clinic.

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Acknowledgments

This study was supported by a special grant for High-Level Training of Yun Nan (D201230) and a special joint grant of Yunnan Provincial Science and Technology Department and Kunming Medical University (2013FZ281 and 2010CD157).

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Authors and Affiliations

  1. Department of Neurosurgery, The First Affiliated Hospital of Kunming Medical University, 295 XiChang Rd, Kunming, 650032, People’s Republic of China

    Peng Ding, Weimin Wang, Jinkun Wang & Zhiyong Yang

  2. Department of Ophthalmology, Yunnan NO. 2 Provincial People’s Hospital, 176 Qingnian Rd, Kunming, 650021, People’s Republic of China

    Liping Xue

Authors
  1. Peng Ding
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  2. Weimin Wang
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  3. Jinkun Wang
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  4. Zhiyong Yang
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  5. Liping Xue
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Corresponding author

Correspondence to Liping Xue.

Additional information

Peng Ding and WeiMin Wang contributed equally to this study.

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Ding, P., Wang, W., Wang, J. et al. Expression of Tumor-Associated Macrophage in Progression of Human Glioma. Cell Biochem Biophys 70, 1625–1631 (2014). https://doi.org/10.1007/s12013-014-0105-3

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  • DOI: https://doi.org/10.1007/s12013-014-0105-3

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