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A Proteomic Analysis of Placental Trophoblastic Cells in Preeclampsia–Eclampsia

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Abstract

To explore the proteomic changes of placental trophoblastic cells in preeclampsia–eclampsia (PE), placental trophoblastic cells from normally pregnant women and women with hypertension during gestational period were prepared by laser capture microdissection (LCM), and proteins isolated from these cells were subjected to labeling and proteolysis with isotope-coded affinity tag reagent. A qualitative and quantitative analysis of the proteome expression of placental trophoblastic cells was made using two-dimensional liquid chromatography tandem mass spectrometry (2D LC–MS/MS). A total of 831 proteins in placental trophoblastic cells were identified by combined use of LCM technique and 2D LC–MS/MS. The result was superior to that of conventional two-dimensional electrophoresis method. There were marked differences in 169 proteins of placental trophoblastic cells between normally pregnant women and women with PE. Of 70 (41.4 %) proteins with more than twofold differences, 31 proteins were down-regulated, and 39 were up-regulated in placental trophoblastic cells of the woman with PE. Laminin expression in placenta trophoblastic cells of women with PE was significantly down-regulated as confirmed by Western blot analysis. These findings provide insights into the proteomic changes in placental trophoblastic cells in response to PE and may identify novel protein targets associated with the pathogenesis of PE.

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Acknowledgments

This research was supported by the National Nature Science Foundation of China (Grant No. 3060075) and the Foundation of China postdoctoral Science (Grant No. 20060400147) and the Foundation of Shanghai postdoctoral, China.

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Authors and Affiliations

  1. Department of Obstetrics & Gynecology, Shandong Provincial Hospital, Shandong University, Jinan, China

    Kaidong Ma, Yan Cheng & Xiaotian Li

  2. The Obstetrics & Gynecology Hospital, Fudan University, 419 Fangxie Road, Shanghai, 200011, China

    Kaidong Ma, Rong Hu, Yu Xiong, Peng Ting & Xiaotian Li

  3. Department of Chemistry, Fudan University, 220 Handan Road, Shanghai, 200433, China

    Hong Jin, Yan Yang & Pengyuan Yang

  4. Institute of Biomedical Science, Fudan University, Shanghai, China

    Hong Jin, Yan Yang & Pengyuan Yang

  5. School of Life Sciences, Queensland University of Technology, 2 George Street, Brisbane, QLD, 4001, Australia

    Shufeng Zhou

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  1. Kaidong Ma
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Correspondence to Kaidong Ma, Pengyuan Yang or Xiaotian Li.

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Kaidong Ma and Hong Jin have contributed equally to this work.

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Ma, K., Jin, H., Hu, R. et al. A Proteomic Analysis of Placental Trophoblastic Cells in Preeclampsia–Eclampsia. Cell Biochem Biophys 69, 247–258 (2014). https://doi.org/10.1007/s12013-013-9792-4

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