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Studies on HOXB4 Expression During Differentiation of Human Cytomegalovirus-infected Hematopoietic Stem Cells into Lymphocyte and Erythrocyte Progenitor Cells

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Abstract

We investigated the role of homeobox B4 (HOXB4) mRNA/protein expression induced by human cytomegalovirus (HCMV) and/or all-trans retinoic acid (ATRA) in proliferation and committed differentiation of human cord blood hematopoietic stem cells (HSCs) into colony-forming-units of T-lymphocyte (CFU-TL) and erythroid (CFU-E) progenitors in vitro. Twelve cord blood samples were collected from the fetal placenta umbilical vein and cultured in vitro. The proliferation and differentiation of cord blood HSCs into CFU-TL and CFU-E were continuously disrupted with HCMV-AD169 and/or 6 × 10−8 mol/l of ATRA. HOXB4 mRNA/protein expression in CFU-TL and CFU-E was detected in control, ATRA, HCMV and ATRA + HCMV groups on days 3, 7, and 12 of culture by fluorescent qRT-PCR/western blot. We found that HOXB4 mRNA/protein expression was detectable on day 3, increased on day 7 and was highest on day 12. HOXB4 mRNA/protein expression in HCMV group was downregulated compared with control group (P < 0.05). However, the levels were significantly upregulated in HCMV + ATRA group compared with HCMV group (P < 0.05). We concluded that the abnormal HOXB4 mRNA/protein expression induced by HCMV could play a role in hematopoietic damage. ATRA, at the concentration used, significantly up-regulated HOXB4 mRNA/protein expression in normal lymphocyte and erythrocyte progenitor cells as well as in HCMV-infected cells.

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Acknowledgments

We thank the Health Bureau of Sichuan Province for financial support (Grant No. 20060040).

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Authors and Affiliations

  1. Department of Pediatrics, Affiliated Hospital of Luzhou Medical College, Luzhou, 646000, Sichuan Province, China

    Liu Wen-jun, Guo qu-lian, Chen Hong-ying, Zou Yan & Huang Mei-xian

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  1. Liu Wen-jun
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  2. Guo qu-lian
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  3. Chen Hong-ying
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  4. Zou Yan
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  5. Huang Mei-xian
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Correspondence to Liu Wen-jun.

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Wen-jun, L., qu-lian, G., Hong-ying, C. et al. Studies on HOXB4 Expression During Differentiation of Human Cytomegalovirus-infected Hematopoietic Stem Cells into Lymphocyte and Erythrocyte Progenitor Cells. Cell Biochem Biophys 63, 133–141 (2012). https://doi.org/10.1007/s12013-012-9349-y

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