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Cardioprotective Efficacy of a Novel Antioxidant Mix VitaePro Against Ex Vivo Myocardial Ischemia–Reperfusion Injury

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Abstract

Circumstantial evidence frequently implicates oxygen-derived free radicals and oxidative stress as mediators of myocardial ischemia/reperfusion (I/R) injury. Therefore, external supplementation of natural antioxidants plays a main role as cardioprotective compounds. This study was designed to evaluate the cardioprotective effect of VitaePro (70 mg/kg body weight, 21 days), a novel antioxidant mix of astaxanthin, lutein and zeaxanthin in a rat ex vivo model of ischemia/reperfusion injury. The cardioprotective effect of VitaePro was also compared with vitamin E (70 mg/kg body weight, 21 days) treatment. Rats were randomized into control I/R (CIR), VitaePro I/R (VPIR) and Vitamin E I/R (VEIR). After 21 days of oral treatment, isolated hearts from each group were subjected to 30 min of ischemia followed by 2 h of reperfusion. In the VPIR group compared to CIR and VEIR groups at 2 h of reperfusion, increased left ventricular functional recovery, such as left ventricular developed pressure (92.7 ± 0.7 vs. 85.3 ± 0.3 and 89.4 ± 1.2 mm Hg), dp/dt max (2518.7 ± 77.9 vs. 1962.5 ± 24 and 2255.7 ± 126.6 mm Hg/s), and aortic flow (21.5 ± 1.36 vs. 4.4 ± 0.6 and 13.2 ± 1.02 ml/min) were observed. The infarct size (27.68 ± 1.7 vs. 45.4 ± 1.8 and 35.4 ± 0.6%), apoptotic cardiomyocytes (61.7 ± 10.6 vs. 194.1 ± 14.8 and 118.7 ± 15.4 counts/100 HPF) and thiobarbituric acid reactive substances levels (80 ± 3 vs. 127 ± 5 and 103 ± 2 nM/mg tissue) also were decreased in VPIR group when compared to CIR and VEIR. As evidenced by the data, administration of vitamin E offered substantial cardioprotection to I/R injury, but VitaePro enhanced cardioprotection significantly more than vitamin E treatment. Taken in concert, the results of this study suggests that the oral ingestion of VitaePro protects myocardium from ischemia/reperfusion injury by decreasing oxidative stress and apoptosis, which may be of therapeutic benefit in the treatment of cardiovascular complications. However, further in vivo animal and human intervention studies are warranted before establishing any recommendations about usage of VitaePro for human cardiovascular complications.

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References

  1. Moens, A. L., Claeys, M. J., Timmermans, J. P., & Vrints, C. J. (2005). Myocardial ischemia/reperfusion-injury, a clinical view on a complex pathophysiological process. International Journal of Cardiology, 100, 179–190.

    Article  PubMed  CAS  Google Scholar 

  2. Saeed, S. A., Waqar, M. A., Zubairi, A. J., Bhurgri, H., Khan, A., Gowani, S. A., et al. (2005). Myocardial ischaemia and reperfusion injury: Reactive oxygen species and the role of neutrophil. Journal of College of Physicians and Surgeons Pakistan, 15, 507–514.

    Google Scholar 

  3. Zweier, J. L., & Talukder, M. A. (2006). The role of oxidants and free radicals in reperfusion injury. Cardiovascular Research, 70, 181–190.

    Article  PubMed  CAS  Google Scholar 

  4. Dhalla, N. S., Elmoselhi, A. B., Hata, T., & Makino, N. (2000). Status of myocardial antioxidants in ischemia–reperfusion injury. Cardiovascular Research, 47, 446–456.

    Article  PubMed  CAS  Google Scholar 

  5. Zhang, X., Klein, A. L., Alberle, N. S., Norby, F. L., Ren, B. H., Duan, J., et al. (2003). Cardiac-specific overexpression of catalase rescues ventricular myocytes from ethanol-induced cardiac contractile defect. Journal of Molecular and Cellular Cardiology, 35, 645–652.

    Article  PubMed  CAS  Google Scholar 

  6. Osganian, S. K., Stampfer, M. J., Rimm, E., Spiegelman, D., Manson, J. E., & Willett, W. C. (2003). Dietary carotenoids and risk of coronary artery disease in women. American Journal of Clinical Nutrition, 77, 1390–1399.

    PubMed  CAS  Google Scholar 

  7. Otton, R., Marin, D. P., Bolin, A. P., Santos, R. D., Polotow, T. G., Sampaio, S. C., et al. (2010). Astaxanthin ameliorates the redox imbalance in lymphocytes of experimental diabetic rats. Chemico-Biological Interactions, 186, 306–315.

    Article  PubMed  CAS  Google Scholar 

  8. Higuera-Ciapara, I., Felix-Valenzuela, L., & Goycoolea, F. M. (2006). Astaxanthin: A review of its chemistry and applications. Critical Reviews in Food Science and Nutrition, 46, 185–196.

    Article  PubMed  CAS  Google Scholar 

  9. Hussein, G., Nakagawa, T., Goto, H., Shimada, Y., Matsumoto, K., Sankawa, U., et al. (2007). Astaxanthin ameliorates features of metabolic syndrome in SHR/NDmcr-cp. Life Sciences, 80, 522–529.

    Article  PubMed  CAS  Google Scholar 

  10. Voutilainen, S., Nurmi, T., Mursu, J., & Rissanen, T. H. (2006). Carotenoids and cardiovascular health. American Journal of Clinical Nutrition, 83, 1265–1271.

    PubMed  CAS  Google Scholar 

  11. Chamberlain, S. M., Hall, J. D., Patel, J., Lee, J. R., Marcus, D. M., Sridhar, S., et al. (2009). Protective effects of the carotenoid zeaxanthin in experimental nonalcoholic steatohepatitis. Digestive Diseases and Sciences, 54, 1460–1464.

    Article  PubMed  CAS  Google Scholar 

  12. Arnal, E., Miranda, M., Barcia, J., Bosch-Morell, F., & Romero, F. J. (2010). Lutein and docosahexaenoic acid prevent cortex lipid peroxidation in streptozotocin-induced diabetic rat cerebral cortex. Neuroscience, 166, 271–278.

    Article  PubMed  CAS  Google Scholar 

  13. Thirunavukkarasu, M., Penumathsa, S. V., Koneru, S., Juhasz, B., Zhan, L., Otani, H., et al. (2007). Resveratrol alleviates cardiac dysfunction in streptozotocin-induced diabetes: Role of nitric oxide, thioredoxin, and heme oxygenase. Free Radical Biology and Medicine, 43, 720–729.

    Article  PubMed  CAS  Google Scholar 

  14. Penumathsa, S. V., Thirunavukkarasu, M., Samuel, S. M., Zhan, L., Maulik, G., Bagchi, M., et al. (2009). Niacin bound chromium treatment induces myocardial Glut-4 translocation and caveolar interaction via Akt, AMPK and eNOS phosphorylation in streptozotocin induced diabetic rats after ischemia-reperfusion injury. Biochimica et Biophysica Acta, 1792, 39–48.

    Article  PubMed  CAS  Google Scholar 

  15. Niehaus, W. G., Jr., & Samuelsson, B. (1968). Formation of malonaldehyde from phospholipid arachidonate during microsomal lipid peroxidation. European Journal of Biochemistry, 6, 126–130.

    Article  PubMed  CAS  Google Scholar 

  16. Mohan, I. K., Khan, M., Wisel, S., Selvendiran, K., Sridhar, A., Carnes, C. A., et al. (2009). Cardioprotection by HO-4038, a novel verapamil derivative, targeted against ischemia and reperfusion-mediated acute myocardial infarction. American Journal of Physiology Heart and Circulatory Physiology, 296, H140–H151.

    Article  PubMed  CAS  Google Scholar 

  17. Thirunavukkarasu, M., Penumathsa, S. V., Samuel, S. M., Akita, Y., Zhan, L., Bertelli, A. A., et al. (2008). White wine induced cardioprotection against ischemia–reperfusion injury is mediated by life extending Akt/FOXO3a/NFkappaB survival pathway. Journal of Agricultural and Food Chemistry, 56, 6733–6739.

    Article  PubMed  CAS  Google Scholar 

  18. Foadoddini, M., Esmailidehaj, M., Mehrani, H., Sadraei, S. H., Golmanesh, L., Wahhabaghai, H., et al. (2010). Pretreatment with hyperoxia reduces in vivo infarct size and cell death by apoptosis with an early and delayed phase of protection. European Journal of Cardio-Thoracic Surgery, 39, 233–240.

    Article  PubMed  Google Scholar 

  19. Ribaya-Mercado, J. D., & Blumberg, J. B. (2004). Lutein and zeaxanthin and their potential roles in disease prevention. Journal of the American College of Nutrition, 23, 567–587.

    Article  Google Scholar 

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Acknowledgment

This study was supported by VitaeLab AS, Enebakkveien 117, 0680 Oslo, Norway.

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Authors and Affiliations

  1. Molecular Cardiology and Angiogenesis Laboratory, Department of Surgery, University of Connecticut School of Medicine, Farmington Avenue, Farmington, CT, 06032, USA

    Ram Sudheer Adluri, Mahesh Thirunavukkarasu, Lijun Zhan & Nilanjana Maulik

  2. Department of Radiology, Harvard Medical School, Boston, MA, USA

    Gautam Maulik

  3. NutriToday, Boston, MA, USA

    Manashi Bagchi

  4. VitaeLab AS, Oslo, Norway

    Katja Svennevig

Authors
  1. Ram Sudheer Adluri
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  2. Mahesh Thirunavukkarasu
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  3. Lijun Zhan
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  4. Nilanjana Maulik
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  5. Katja Svennevig
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  6. Manashi Bagchi
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  7. Gautam Maulik
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Correspondence to Gautam Maulik.

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Adluri, R.S., Thirunavukkarasu, M., Zhan, L. et al. Cardioprotective Efficacy of a Novel Antioxidant Mix VitaePro Against Ex Vivo Myocardial Ischemia–Reperfusion Injury. Cell Biochem Biophys 67, 281–286 (2013). https://doi.org/10.1007/s12013-011-9300-7

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  • DOI: https://doi.org/10.1007/s12013-011-9300-7

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