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In Vitro Model of Bacterial Biofilm Formation on Polyvinyl Chloride Biomaterial

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Abstract

The aim of the study was to establish an in vitro model of Staphylococcus epidermidis biofilms on polyvinyl chloride (PVC) material, and to investigate bacterial biofilm formation and its structure using the combined approach of confocal laser scanning microscope (CLSM) and scanning electron microscope (SEM). Staphylococcus epidermidis bacteria (stain RP62A) were incubated with PVC pieces in Tris buffered saline to form biofilms. Biofilm formation was examined at 6, 12, 18, 24, 30, and 48 h. Thicknesses of these biofilms and the number, and percentage of viable cells in biofilms were measured. CT scan images of biofilms were obtained using CLSM and environmental SEM. The results of this study showed that Staphylococcus epidermidis biofilm is a highly organized multi-cellular structure. The biofilm is constituted of large number of viable and dead bacterial cells. Bacterial biofilm formation on the surface of PVC material was found to be a dynamic process with maximal thickness being attained at 12–18 h. These biofilms became mature by 24 h. There was significant difference in the percentage of viable cells along with interior, middle, and outer layers of biofilms (P < 0.05). Staphylococcus epidermidis biofilm is sophisticated in structure and the combination method involving CLSM and SEM was ideal for investigation of biofilms on PVC material.

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References

  1. Kadurugamuwa, J. L., Sin, L., Albert, E., et al. (2003). Direct continuous method for monitoring biofilm infection in a mouse model. Infection and Immunity, 71(2), 882–890.

    Article  PubMed  CAS  Google Scholar 

  2. Huang, Y.-c., ZHANG, E.-y., Shi, Y.-k., et al. (2004). Study on bacteria adhesion to prosthetic valve materials and the eliminating activity in rabbit body. Biomedical Engineering and Clinical Medicine, 8(2), 61–64.

    Google Scholar 

  3. Li, Y.-x., Huang, Y.-c., Yang, D.-k., et al. (2003). The effect of human serum albumin on bacterial adhesion to cardiovascular biomaterials in vitro. Chinese Journal of Clinical Thoracic and Cardiovascular Surgery, 10(4), 276–279.

    Google Scholar 

  4. Li, J., Wang, J., Shen, L., et al. (2006). Antibacterial characteristics of polyethylene terephthalate surface-modified by silver ion implantation. Chinese Journal of Vacuum Science and Technology, 26(5), 408–412.

    Article  Google Scholar 

  5. Rais-Bahrami, K., Nunez, S., Revenis, M. E., et al. (2004). Follow-up study of adolescents exposed to di(2-ethylhexyl) phthalate (DEHP) as neonates on extracorporeal membrane oxygenation (ECMO) support. Environmental Health Perspectives, 112(13), 1339–1340.

    Article  PubMed  CAS  Google Scholar 

  6. Huang, Y.-c., Yang, K.-y., Lei, Y.-j., et al. (2008). Relationship between bacterial adhesion to prosthetic valve materials and bacterial growth. Journal of Clinical Rehabilitative Tissue Engineering Research, 12, 112–115.

    Google Scholar 

  7. Coenye, T., & Nelis, H. J. (2010). In vitro and in vivo model systems to study microbial biofilm formation. Journal of Microbiological Methods, 83(2), 89–105.

    Article  PubMed  CAS  Google Scholar 

  8. Del Pozo, J. L., & Patel, R. (2007). The challenge of treating biofilm-associated bacterial infections. Clinical Pharmacology and Therapeutics, 82(2), 204–209.

    Article  PubMed  CAS  Google Scholar 

  9. Huang, Y.-c., Zhang, E.-y., & Shi, Y.-k. (1999). The study advancement of bacterial adhesion on biomaterials. Foreign Medicine Biological Engineering Fascicle, 22(3), 148–152.

    Google Scholar 

  10. Hope, C. K., Clements, D., & Wilson, M. (2002). Determining the spatial distribution of viable and nonviable bacteria in hydrated microcosm dental plaques by viability profiling. Applied Microbiology, 93(2), 448–455.

    Article  CAS  Google Scholar 

  11. Impenny, J., Manz, W., & Szewzky, V. (2000). Heterogencity in biofilms. FEMS Microbiology Reviews, 24(5), 661–671.

    Article  Google Scholar 

Download references

Acknowledgment

This study was partly supported by the grants from the National Natural Science Foundation of China (No.30872555, 81000672), Yunnan Provincial Science Foundation (No.2008ZC139 M, 2009CD184).

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Authors and Affiliations

  1. Department of Cardiothoracic Surgery, Third Affiliated Hospital of Kunming Medical University (Tumor Hospital of Yunnan Province), 519 Kun-Zhou Road, Kunming, 650118, Yunnan, China

    Guang-qiang Zhao, Lian-hua Ye, Yun-chao Huang & Yu-jie Lei

  2. Department of Cardiothoracic Surgery, Second Affiliated Hospital of Kunming Medical University, Kunming, China

    Da-kuan Yang

  3. Biological Therapy Center, No.3 Affiliated Hospital of Kunming Medical University, Kunming, 650118, Yunnan, China

    Li Li

  4. Department of Cardiothoracic Surgery, Shangdong Heze Hospital, Heze, 274031, Shandong, China

    Geng Xu

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  1. Guang-qiang Zhao
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  2. Lian-hua Ye
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  3. Yun-chao Huang
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  4. Da-kuan Yang
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  5. Li Li
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  7. Yu-jie Lei
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Correspondence to Yun-chao Huang.

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Zhao, Gq., Ye, Lh., Huang, Yc. et al. In Vitro Model of Bacterial Biofilm Formation on Polyvinyl Chloride Biomaterial. Cell Biochem Biophys 61, 371–376 (2011). https://doi.org/10.1007/s12013-011-9220-6

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  • DOI: https://doi.org/10.1007/s12013-011-9220-6

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