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Profile of Exoglycosidases in Synovial Cell Cultures Derived from Human Synovial Membrane

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Abstract

Objective Determining the activity of lysosomal exoglycosidases in tissue cultures of synoviocytes derived from the knee joints of patients with injured anterior cruciate ligaments (ACL), juvenile idiopathic arthritis (JIA), and rheumatoid arthritis (RA). Methods The following exoglycosidases in cultured synoviocytes were analyzed with p-nitrophenyl derivatives of appropriate sugars as substrates: hexosaminidase (HEX) and its isoenzyme A (HEX-A), β-glucuronidase (GluA), β-galactosidase (GAL), α-mannosidase (MAN), and α-fucosidase (FUC). Results In our cell cultures, fibroblast-like synovial cells (FLS) dominated. In the group of patients with ACL-injuries, and in the groups of patients with JIA and RA, the activity of the investigated exoglycosidases was significantly higher in the intra- rather than in the extracellular compartment. Hexosaminidase was the predominant exoglycosidase. Stimulation of synoviocytes by IL-1β in cell cultures significantly increased the activity of HEX, HEX-A, and GluA in both compartments, as well as of GAL, MAN, and FUC in the intracellular compartment. Stimulation by IL-1β rheumatoidal synoviocytes increased by 128–201% the activity of HEX and HEX A in intracellular compartments and 33–72% in extracellular compartment. Conclusions The profile of lysosomal exoglycosidases in a cell culture of human synoviocytes is similar, but not identical, to those in the knee joint. Hexosaminidase is the dominant glycosidase in cultured unstimulated and IL-1β-stimulated human synoviocytes. The HEX inhibitors may be new drugs for the treatment of inflamed knee joints.

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Authors and Affiliations

  1. Department of Pediatric Orthopedics and Traumatology, Medical University, Bialystok, Poland

    Janusz Popko, Justyna Marciniak & Tomasz Guszczyn

  2. Department of Pediatric Orthopedics and Traumatology, Children’s Hospital, Waszyngtona 17 Str., Bialystok, 15-269, Poland

    Janusz Popko

  3. Department of Pharmaceutical Biochemistry, Medical University, Bialystok, Poland

    Justyna Marciniak, Malgorzata Knas & Krzysztof Zwierz

  4. Department of Cytogenetics, Medical University, Bialystok, Poland

    Elzbieta Ilendo & Jolanta Wysocka

  5. Department of Pediatric Alergology, Medical University, Bialystok, Poland

    Anna Stasiak-Barmuta

  6. Department of Respiratory Diagnostics and Bronchofiberoscopy, Medical University, Bialystok, Poland

    Tadeusz Moniuszko

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  1. Janusz Popko
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  2. Justyna Marciniak
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  9. Jolanta Wysocka
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Correspondence to Janusz Popko.

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Popko, J., Marciniak, J., Ilendo, E. et al. Profile of Exoglycosidases in Synovial Cell Cultures Derived from Human Synovial Membrane. Cell Biochem Biophys 51, 89–95 (2008). https://doi.org/10.1007/s12013-008-9018-3

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  • DOI: https://doi.org/10.1007/s12013-008-9018-3

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