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Impact of LPS-Induced Cardiomyoblast Cell Apoptosis Inhibited by Earthworm Extracts

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Abstract

Dilong is an earthworm extract with a dense nutritional content, widely used in Chinese herbal medicine to remove stasis and stimulate wound healing. Earthworm extracts are traditionally used by indigenous people throughout the world. How this Dilong inhibits Lipopolysaccharide (LPS)-induced cardiomyoblast cell apoptosis is still unclear. This study investigates the Dilong extract effect on LPS-induced apoptosis in H9c2 cardiomyoblast cells. LPS (1 μg/ml) administration for 24 h induced apoptosis in H9c2 cells. Cell apoptosis was detected using MTT, LDH, TUNEL assay and JC-1 staining. Western blot analysis was used to detect pro-apoptotic and anti-apoptotic proteins. Dilong extract totally blocked the LPS impact, leading to the activation of anti-apoptotic proteins, Bcl-2 and Bcl-xL, stabilized the mitochondria membrane and down-regulated the extrinsic and intrinsic pro-apoptotic proteins, TNF-α, active caspase-8, t-Bid, Bax, active caspase-9 and active caspase-3. Dilong could potentially serve as a cardio protective agent against LPS-induced H9c2 cardiomyoblast cell apoptosis.

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Acknowledgments

This study is supported by the Taiwan Department of Health Clinical Trial and Research Center for Excellence (DOH102-TD-B-111-004) and in part by (CMU99-EW-05, CMU102-BC-6) China Medical University.

Author information

Authors and Affiliations

  1. Division of Cardiovascular Surgery, China Medical University Hospital, Taichung, Taiwan

    Ping-Chun Li

  2. Department of Life Science, Tunghai University, Taichung, Taiwan

    Ping-Chun Li

  3. Graduate Institute of Chinese Pharmaceutical Sciences, China Medical University, Taichung, Taiwan

    Yun-Chen Tien

  4. Department of Nursing, MeiHo University, Pingtung, Taiwan

    Cecilia Hsuan Day

  5. Division of Cardiology, China Medical University Hospital, Taichung, Taiwan

    Peiying Pai

  6. Department of Biological Science and Technology, China Medical University, Taichung, Taiwan

    Wei-Wen Kuo

  7. Graduate Institute of Basic Medical Science, China Medical University, No 91, Hsueh-Shih Road, Taichung, 404, Taiwan

    Tung-Sheng Chen & Chih-Yang Huang

  8. Department of Sports Sciences, University of Taipei, Taipei, Taiwan

    Chia-Hua Kuo

  9. Department of Healthcare Administration, Asia University, Taichung, Taiwan

    Chang-Hai Tsai

  10. Department of Neurological Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan

    Da-Tong Ju

  11. Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan

    Chih-Yang Huang

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  1. Ping-Chun Li
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  2. Yun-Chen Tien
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Correspondence to Chih-Yang Huang.

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Li, PC., Tien, YC., Day, C.H. et al. Impact of LPS-Induced Cardiomyoblast Cell Apoptosis Inhibited by Earthworm Extracts. Cardiovasc Toxicol 15, 172–179 (2015). https://doi.org/10.1007/s12012-014-9281-z

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  • DOI: https://doi.org/10.1007/s12012-014-9281-z

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