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Potentially Reduced Exposure Cigarettes Accelerate Atherosclerosis: Evidence for the Role of Nicotine

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Abstract

The tobacco industry markets potentially reduced exposure products (PREPs) as less harmful or addictive alternatives to conventional cigarettes. This study compared the effects of mainstream smoke from Quest, Eclipse, and 2R4F reference cigarettes on the development of atherosclerosis in apolipoprotein E-deficient (apoE −/−) mice. Mice were exposed to smoke from four cigarette types for 12 weeks beginning at age of 12 weeks, and in a separate study for 8 weeks, beginning at age of 8 weeks. In both studies, mice exposed to smoke from high-nicotine, high-tar Quest 1, and 2R4F cigarettes developed greater areas of lipid-rich aortic lesions than did non-smoking controls. Exposure to smoke from the lower-nicotine products, Eclipse, and Quest 3, was associated with smaller lesion areas, but animals exposed to smoke from all of the tested types of cigarette had larger lesions than did control animals not exposed to smoke. Urinary levels of isoprostane F2 alpha VI, increased proportionally to cigarette nicotine yield, whereas induction of pulmonary cytochrome P4501A1 was proportional to tar yield. Lesion area was associated with both nicotine and tar yields, although in multiple regression analysis only nicotine was a significant predictor of lesion area. Smoke exposure did not alter systolic blood pressure (SBP), heart rate (HR), blood cholesterol, or leukocyte count. Taken together, these observations suggest that smoking may accelerate atherosclerosis by increasing oxidative stress mediated at least in part via the actions of nicotine.

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Acknowledgements

We thank Dr. Stephen S. Hecht for carrying out measurements of urinary nicotine and cotinine, and Dr. Sharon Murphy for helpful comments on the manuscript. This work was supported in part by NIH grants HL 64660 to Daniel F Catanzaro and HL 55627 to Babette B. Weksler.

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Authors and Affiliations

  1. Department of Cardiothoracic Surgery, Weill Medical College of Cornell University and New York Presbyterian Hospital, 1300 York Avenue, P.O. Box 118, New York, NY, 10021, USA

    Daniel F. Catanzaro, Ying Zhou, Rong Chen, Fangmin Yu & Sarah E. Catanzaro

  2. Department of Medicine, Weill Medical College of Cornell University and New York Presbyterian Hospital, New York, NY, 10021, USA

    Daniel F. Catanzaro, Mariana S. De Lorenzo, Kotha Subbaramaiah, Andrew J. Dannenberg & Babette B. Weksler

  3. Department of Public Health, Weill Medical College of Cornell University and New York Presbyterian Hospital, New York, NY, 10021, USA

    Xi Kathy Zhou

  4. Department of Pharmacology, University of Pennsylvania, School of Medicine, Philadelphia, PA, 19104-6059, USA

    Domenico Pratico

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  1. Daniel F. Catanzaro
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Correspondence to Daniel F. Catanzaro.

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Catanzaro, D.F., Zhou, Y., Chen, R. et al. Potentially Reduced Exposure Cigarettes Accelerate Atherosclerosis: Evidence for the Role of Nicotine. Cardiovasc Toxicol 7, 192–201 (2007). https://doi.org/10.1007/s12012-007-0027-z

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