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Alleviating Effect of Methionine on Intestinal Development and Intercellular Junction Induced by Nickel

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Abstract

The aim of this study was to investigate the alleviating effect of methionine (Met) on intestinal injury induced by nickel. The mice were divided into six groups: Met-deficient + nickel group (MDN), Met-deficient group (MD), Met + nickel group (MN), high-dose Met + nickel group (HMN), high-dose Met group (HM), and blank control group (BC). Histopathological techniques, Alcian blue-periodic acid Schiff (AB-PAS) staining, enzyme-linked immunosorbent assay (ELISA), and real-time PCR were used to study the changes of intestinal development, the number of goblet cells, and the intercellular junction. The results showed that Met can inhibit the intestinal villus length and crypt depth decreases induced by nickel and increase the index villus length and crypt depth (V/C), the number of goblet cells, and the content of diamine oxidase (DAO) and decrease the content of fatty acid binding protein2 (FABP2) and endotoxin (ET) of the intestinal mucosa damage parameters, and the mRNA expression of intercellular junction (occludin, ZO-1, claudin-1) was damaged. It is suggested that Met could help inhibit the toxic effect of nickel on the intestinal development and intercellular connection.

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Acknowledgements

The authors would like to thank the co-workers of China West Normal University for their assistance in performing the experiments and analysis.

Funding

This study is supported by the program for the Fundamental Research Funds of China West Normal University (project no. 20A003), the Meritocracy Research Funds of China West Normal University (project no. 17YC349), and the Innovation Training Program for College Students (project no. S202110638058).

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Authors and Affiliations

  1. Key Laboratory of Southwest China Wildlife Resources Conservation, Ministry of Education People’s Republic of China, Nanchong, People’s Republic of China

    Bangyuan Wu

  2. College of Life Sciences, China West Normal University, Nanchong, People’s Republic of China

    Bangyuan Wu, Yongci Tan, Haiying Huang, Yiwei Liu, Tingrui Bai & Lulu Yang

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  1. Bangyuan Wu
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Correspondence to Bangyuan Wu.

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All animal experiments were carried out in accordance with the Animal Welfare Committee of China West Normal University under the guidance of the Chinese government regulations.

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Wu, B., Tan, Y., Huang, H. et al. Alleviating Effect of Methionine on Intestinal Development and Intercellular Junction Induced by Nickel. Biol Trace Elem Res 200, 4007–4016 (2022). https://doi.org/10.1007/s12011-021-02992-9

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