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Effect of Iodine and Selenium on Proliferation, Viability, and Oxidative Stress in HTR-8/SVneo Placental Cells

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Abstract

Adequate maternal micronutrition is vital for placental formation, fetal growth, and development. Oxidative stress adversely affects placental development and function and an association between deficient placental development, oxidative stress, and micronutrient deficiency has been observed. Selenium and iodine are two essential micronutrients with antioxidant properties. Epidemiological studies have shown that poor micronutrient status in pregnant women is associated with a higher incidence of pregnancy complications. The aim of this study was to determine how selenium, iodine, and their combination impact oxidative stress in placental trophoblast cells. HTR8/SVneo extravillous trophoblasts were supplemented with a concentration range of organic and inorganic selenium, potassium iodide, or their combination for 24 h. Oxidative stress was then induced by treating cells with menadione or H2O2 for 24 h. Cell viability and lipid peroxidation as the biomarker of oxidative stress were assessed at 48 h. Both menadione and H2O2 reduced cell viability and increased lipid peroxidation (P < 0.05). Greater cell viability was found in selenium-supplemented cells when compared with vehicle treated cells (P < 0.05). Selenium and iodine supplementation separately or together were associated with lower lipid peroxidation compared with vehicle control (P < 0.05). Supplementation with the combination of selenium and iodine resulted in a greater reduction in lipid peroxidation compared with selenium or iodine alone (P < 0.05). Oxidative stress negatively impacts trophoblast cell survival and cellular integrity. Selenium and iodine protect placental trophoblasts against oxidative stress. Further research is warranted to investigate the molecular mechanisms by which selenium and iodine act in the human placenta.

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Funding

This research has been supported by National Health and Medical Research Council (NHMRC) GNT1161079 Targeting micronutrients to tackle pregnancy disorders: an integrated approach awarded to CTR, SJZ, AVP, SYL and TBM. CTR is supported by a NHMRC Investigator Grant GNT1174971 and a Flinders University Matthew Flinders Fellowship.

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Authors and Affiliations

  1. School of Agriculture, Food and Wine, Waite Research Institute, University of Adelaide, Adelaide, SA, 5005, Australia

    Nahal Habibi, Shao Jia Zhou & Tina Bianco-Miotto

  2. Robinson Research Institute, University of Adelaide, Adelaide, SA, 5005, Australia

    Nahal Habibi, Tanja Jankovic-Karasoulos, Shalem Yiner-Lee Leemaqz, Shao Jia Zhou, Claire T. Roberts & Tina Bianco-Miotto

  3. Adelaide Medical School, University of Adelaide, Adelaide, SA, 5005, Australia

    Tanja Jankovic-Karasoulos, Shalem Yiner-Lee Leemaqz & Claire T. Roberts

  4. CSIRO Health and Biosecurity, Future Science Platforms Probing Biosystems, Adelaide, SA, 5000, Australia

    Maxime Francois & Wayne R. Leifert

  5. School of Biological Sciences, University of Adelaide, Adelaide, SA, 5005, Australia

    Maxime Francois & Wayne R. Leifert

  6. School of Medical Science, Griffith University, Gold Coast Campus, Parklands Drive, Southport, QLD, 9726, Australia

    Anthony V. Perkins

  7. College of Medicine and Public Health, Flinders University, Bedford Park, SA, 5042, Australia

    Claire T. Roberts

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  1. Nahal Habibi
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Habibi, N., Jankovic-Karasoulos, T., Leemaqz, S.YL. et al. Effect of Iodine and Selenium on Proliferation, Viability, and Oxidative Stress in HTR-8/SVneo Placental Cells. Biol Trace Elem Res 199, 1332–1344 (2021). https://doi.org/10.1007/s12011-020-02277-7

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