Abstract
Present study was undertaken to investigate the toxic effect of sodium fluoride (NaF)– and fluoride (F)-contaminated ground water on reproductive performances of male rats. Healthy adult male rats were categorised into three groups, first group of rats were served as control, whereas second group of rats were orally intubated with NaF (10 mg/kgbw/1 ml/rat) and third group of rats were allowed to drink F-contaminated ground water (5 mg/L) through drinking water bottles for 52 days. Exposure of NaF- and F-contaminated ground water caused significant decline in sperm motility, serum concentration of testosterone, and increase in sperm abnormality compared with controls. Further, significant histological alterations characterized with shrunken seminiferous tubules and degeneration of different stages of spermatogonial cells were observed in rats treated with NaF- and F-contaminated ground water. After the confirmation of toxic effect of F, these NaF- and F-contaminated ground water–treated male rats were allowed to mate with proven fertile untreated female rats to study the reproductive performances of male rats. There was a decline in parturition index, fertility index of male and female, gestation index and number of pups delivered in NaF-treated male rats compared with controls. However, gestation index and number of pups delivered were declined in F-contaminated ground water–treated male rats compared with controls. These results clearly indicate that F exposure affected the reproductive performances of male rats. The present study further revealed the fact that F-induced decline in testosterone levels, reduced sperm motility, and loss of spermatogonial cells affected the reproductive performances of male rats.
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References
Adilaxmamma K, Janardhan K, Reddy KS (1994) Monocrotophos: reproductive toxicity in rats. Indian J Pharmaco 26:126–129
Al-Hiyasat AS, Elbetieho AM, Darmani H (2000) Reproductive toxic effects of ingestion of sodium fluoride in female rats. Fluoride. 33:9–24
Bataineh H, Nusier M (2006) Impact of 12- week ingestion of sodium fluoride on aggression, sexual behavior, and fertility in adult male rats. Fluoride. 39(4):293–301
Chinoy NJ, Narayana MV, Dalal V, Rawat M, Patel D (1995) Amelioration of fluoride toxicity in some accessory glands and spermatozoa of rat. Fluoride. 28:75–86
Collins T, Sprando R, Black T, Schackleford M, Olejnik N, Ames M, Rorie J, Ruggles D (2001) Developmental toxicity of sodium fluoride measured during multiple generations. Food Chem Toxicol 39:867–876
Darmani H, Al-Hiyasat AS, Elbetieha AM (2001) Effects of sodium fluoride in drinking water on fertility in female mice. Fluoride. 34:242–249
Dhurvey V, Thakare M (2016) The effect of sodium fluoride intoxication on the estrous cycle and ovarian hormones in rats. Fluoride 49:223–232
Elbetieha A, Darmani H, Al-Hiyasat A (2000) Fertility effects of sodium fluoride in male mice. Fluoride. 33:128–134
El-lethey HS, Kamel MM, Shaheed IB (2011) Perinatal exposure to sodium fluoride with emphasis on territorial aggression, sexual behaviour and fertility in male rats. J Life Science 8:686–694
Freni SC (1994) Exposure to high fluoride concentrations in drinking water is associated with decreased birth rates. J Toxicol Environ Health 42:109–121
Forrestier F, Daffos F, Said R, Brunet CM, Guillaume PN (1990) The passage of fluoride across placenta; an intrauterine study. J Gynecol Obstet Biol Reprod 19:171–175
Gadallah A (2016) Teratogenicity of sodium fluoride on newly born rats. JBSAR 2(3):203–207
Gessner BD, Beller M, Middaugh JP, Whitford GM (1994) Acute fluoride poisoning from a public water system. N Engl J Med 330:95–99
Ghosh D, Das S, Maiti R, Jana D, Das UB (2002) Testicular toxicity in sodium fluoride treated rats; association with oxidative stress. Reprod Toxicol 16:385–390
Gunasherlin DM, Verma RJ, Jacob K (1999) Embryotoxicity of fluoride in rat. Indian J Environ Toxicol 9:27–29
Gupta RS, Khan TI, Agarwal D, Kacchawa JBS (2007) The toxic effect of sodium fluoride on the reproductive system of male rats. Toxicol Ind Health 23:507–513
Hiyasat ASA, Elbetieha AM, Darmani H (2000) Reproductive toxic effects of ingestion of sodium fluoride in female rats. Fluoride. 33:79–84
Huang C, Niu R, Wang J (2007) Toxic effects of sodium fluoride on reproductive function in male mice. Fluoride. 40:162–168
Kennedy GL, John JP, Frawley J, Calandra JC (1973) Multi-generation reproductive effects of three pesticides in rats. Toxicol Appl Pharmacol 25:589–596
Kumar A, Susheela A (1994) Ultra-structural studies of spermiogenesis in rabbit exposed to chronic fluoride toxicity. J Dent Res 39:164–171
Kumar A, Susheela A (1995) Effects of chronic fluoride toxicity on the morphology of ductus epididymis and the maturation of spermatozoa of rabbit. Int J Exp Pathol 76:1–11
Kim J, Kwon WS, Rahman MS, Lee JS, Yoon SJ, Park YJ, You YA, Pang MG (2015) Effect of sodium fluoride on male mouse fertility. Andrology 3:544–551
Liang S, Zhao MH, Ock SA, Kim NH and Cui XS (2015) Fluoride impairs oocyte maturation and subsequent embryonic development in mice. EnvironToxicol. 32:1486–1495
Malhotra A, Tewari A, Chawla HS, Gausa K, Dhall K (1993) Placental transfer of fluoride in pregnant women consuming optimum fluoride in drinking water. J Indian Soc Pedod Prev Dent 11:1–3
Narayana M, Chinoy N (1994) Effect of fluoride on rat testicular steroidogenesis. Fluoride. 27:7–12
Narayana K, D’Souza UJA, Rao KPS (2002) Ribavirin-induced sperm shape abnormalities in Wister rat. Mutat Res 513:193–196
Narayana K, Prashanthi N, Nayanatara A, Harish HCK, Abhilash K, Bairy KL (2005) Effects of methyl parathion (o,o-dimethyl o-4- nitrophenylphosphorothioate) on rat sperm morphology and sperm count, but not fertility, are associated with decreased ascorbic acid level in the testis. Mutat Res 588:28–34
Opydo-Szymaczek J, Borysewicz-Lewicka M (2007) Trans-placental passage of fluoride in pregnant polish women assessed in the basis of fluoride concentration. Fluoride. 40:46–50
Pereira PAA, Lichtenfels AJ, Pereira LAA, Malm O, Saldiva PHN, Braga ALF, Lin CA (2013) Effects of environmental water contamination in the reproductive system of mice. J Environ Prot 4:5–125. https://doi.org/10.4236/jep.2013.47A014
Saalu LC, Kpela T, Benebo AS, Oyewopo AO, Anifowope EO, Oguntola JA (2010) The dose-dependent testiculo-protective and testiculo-toxic potentials of Telfairia occidentalis leaves extract in rat. Int J Appl Res Nat Prod 3:27–28
Sengupta P (2013) Environmental and occupational exposure of metals and their role in male reproductive functions. Drug Chem Toxicol 36(3):353–368. https://doi.org/10.3109/01480545.2012.710631
Singh PK, Feroz AD, Sheeba H, Khalil A, Samir AM (2012) Beneficial effect of Tamarindus indica on the testes of albino rat after fluoride intoxication. Int J Pharm Bio Sci 3:487–493
Spittle B (2007) Review of fluoride poisoning: is fluoride in your drinking water and from other sources making you sick? Fluoride. 40:250–252
Susheela AK, Kumar AA (1991) Study of the effect of high concentrations of fluoride on the reproductive organs of male rabbits, using light and scanning electron microscopy. J Reprod Fertil 92:353–360
Susheela A, Jethanandani P (1996) Circulating testosterone levels in skeletal fluorosis patients. J Toxicol Clin Toxicol 34:183–189
Teotia M, Teotia SPS, Singh RK (1979) Metabolism of fluoride in pregnant women residing in endemic fluorosis area. Fluoride. 12:58–64
Theure RC, Mahoney AW, Sarett HP (1971) Placental transfer of fluoride and tin in rats given various fluoride and tin salts. J Nutr 101:525–532
Veeramachaneni DNR, Palmer JS, Amann RP (2001) Long-term effects on male reproduction of early exposureto common chemical contaminants in drinking water. Hum Reprod 16(5):979–987
Vega SG, Guzman P, Garcia L, Espinosa J, de Nava CCE (1988) Sperm shape abnormality and urine mutagenicity in mice treated with niclosamide. Mutat Res 204:269–276
Wan SX, Zhang JH, Wang JD (2006a) Effects of high fluoride on sperm quality in male rats. Fluoride. 39:17–21
Wan SX, Zhang JH, Wang JD (2006b) Fluoride induced changes in the expression of epidermal growth factor and its receptor in testicular tissues of young male rats. Fluoride. 39:121–125
Whitford G (1996) The metabolism and toxicity of fluoride. Second edition. Monogr Oral Sci 2:1–156
Zhang J, Liang C, Ma J, Niu R, Wang J (2006) Effects of sodium fluoride and sulfurdioxide on sperm motility and serum testosterone in male rats. Fluoride. 39:126–131
Zhou T, Duan LJ, Ding Z, Yang RP, Li SH, Xi Y, Cheng XM, Hou JX, Wen SB, Chen J, Cui LX, Ba Y (2012) Environmental fluoride exposure and reproductive hormones in male living in endemic fluorosis villages in china. Life Sci 9:4
Zhou Y, Zhang H, He J, Chen X, Ding Y, Wang Y, Liu X (2013) Effects of sodium fluoride on reproductive function in female rats. Food Chem Toxicol 56:229–231
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Approval for the proposed animal experiments was obtained from Institutional Animal Ethics Committee of University of Mysore, India (Reference number-UOM/IAEC/04/2017). The experimental procedures were performed in accordance with the ethical standards of the Committee for the Purpose of Control and Supervision of Experiments on Animals, India.
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Chaithra, B., Sarjan, H.N. & Shivabasavaiah Sodium Fluoride and Fluoride Contaminated Ground Water Induced Altered Reproductive Performances in Male Rats. Biol Trace Elem Res 195, 544–550 (2020). https://doi.org/10.1007/s12011-019-01882-5
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DOI: https://doi.org/10.1007/s12011-019-01882-5