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Preliminary Study of Mechanisms of Fluoride-Induced Suppression of Nitric Oxide Synthesis in Human Umbilical Vein Endothelial Cells

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Abstract

This study was aimed to determine if excessive exposure to fluoride could suppress the synthesis of nitric oxide (NO) and to detail the mechanisms involved. With the exception of the control group, human umbilical vein endothelial cells (HUVECs) were treated with sodium fluoride (NaF) (1.2 μg/mL) for 24 h, with or without a 2-h pretreatment with 100 nM insulin-like growth factor 1 (IGF-1, a PI3K/AKT agonist), or 10 μM histamine (HIS, a eNOS agonist). The levels of NO in culture fluids, as well as the expressions of eNOS, p-eNOS, PI3K, AKT, and p-AKT, were compared. The levels of NO significantly decreased in all experimental groups; however, the levels of NO were obviously higher in the NaF + HIS and NaF + IGF-1 groups, compared to the NaF group. The p-eNOS/eNOS ratios dropped clearly in NaF and NaF + HIS groups, while that in the NaF + HIS group was distinctly higher than that in the NaF group. The p-AKT/AKT ratios went down apparently in NaF and NaF + IGF-1 groups, while that in the NaF + IGF-1 group was overtly higher than that of the NaF group. Excessive exposure to fluoride inhibited the synthesis of NO. The PI3K/AKT/eNOS pathway played a crucial role in the reduced expression of NO caused by excessive fluoride exposure.

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Authors and Affiliations

  1. Department of Rheumatology and Immunology, the First Hospital, Jilin University, 71# Xinmin Street, Changchun, 130021, China

    Yuanyuan Huang & Zhenyu Jiang

  2. Department of Pediatrics, the First Hospital, Jilin University, Changchun, 130021, China

    Yuanyuan Huang, Meihua Sun, Feng Li & Haibo Li

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  1. Yuanyuan Huang
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  2. Meihua Sun
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  3. Feng Li
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  4. Haibo Li
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  5. Zhenyu Jiang
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Correspondence to Zhenyu Jiang.

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Huang, Y., Sun, M., Li, F. et al. Preliminary Study of Mechanisms of Fluoride-Induced Suppression of Nitric Oxide Synthesis in Human Umbilical Vein Endothelial Cells. Biol Trace Elem Res 185, 311–315 (2018). https://doi.org/10.1007/s12011-018-1252-y

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  • DOI: https://doi.org/10.1007/s12011-018-1252-y

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