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The Effects of Selenium Supplementation on Gene Expression Related to Insulin and Lipid in Infertile Polycystic Ovary Syndrome Women Candidate for In Vitro Fertilization: a Randomized, Double-Blind, Placebo-Controlled Trial

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28 February 2020 The Editors-in-Chief are currently investigating this article [Zadeh Modarres, S., Heidar, Z., Foroozanfard, F. et al. The Effects of Selenium Supplementation on Gene Expression Related to Insulin and Lipid in Infertile Polycystic Ovary Syndrome Women Candidate for In Vitro Fertilization: a Randomized, Double-Blind, Placebo-Controlled Trial. Biol Trace Elem Res 183, 218–225 (2018). https://doi.org/10.1007/s12011-017-1148-2] as concerns have been raised about integrity of the clinical trial reported here. There is also an ongoing investigation by the Iranian National Committee for Ethics in Biomedical Researches. Further editorial action will be taken as appropriate once the investigation into the concerns is complete and all parties have been given an opportunity to respond in full.

Abstract

This study was conducted to evaluate the effects of selenium supplementation on gene expression related to insulin and lipid in infertile women with polycystic ovary syndrome (PCOS) candidate for in vitro fertilization (IVF). This randomized double-blind, placebo-controlled trial was conducted among 40 infertile women with PCOS candidate for IVF. Subjects were randomly allocated into two groups to intake either 200-μg selenium (n = 20) or placebo (n = 20) per day for 8 weeks. Gene expression levels related to insulin and lipid were quantified in lymphocytes of women with PCOS candidate for IVF with RT-PCR method. Results of RT-PCR demonstrated that after the 8-week intervention, compared with the placebo, selenium supplementation upregulated gene expression of peroxisome proliferator-activated receptor gamma (PPAR-γ) (1.06 ± 0.15-fold increase vs. 0.94 ± 0.18-fold reduction, P = 0.02) and glucose transporter 1 (GLUT-1) (1.07 ± 0.20-fold increase vs. 0.87 ± 0.18-fold reduction, P = 0.003) in lymphocytes of women with PCOS candidate for IVF. In addition, compared with the placebo, selenium supplementation downregulated gene expression of low-density lipoprotein receptor (LDLR) (0.88 ± 0.17-fold reduction vs. 1.05 ± 0.22-fold increase, P = 0.01) in lymphocytes of women with PCOS candidate for IVF. We did not observe any significant effect of selenium supplementation on gene expression levels of lipoprotein(a) [LP(a)] in lymphocytes of women with PCOS candidate for IVF. Overall, selenium supplementation for 8 weeks in lymphocytes of women with infertile PCOS candidate for IVF significantly increased gene expression levels of PPAR-γ and GLUT-1 and significantly decreased gene expression levels of LDLR, but did not affect LP(a).

Clinical trial registration number: http://www.irct.ir: IRCT201704245623N113.

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  • 28 February 2020

    The Editors-in-Chief are currently investigating this article [Zadeh Modarres, S., Heidar, Z., Foroozanfard, F. et al. The Effects of Selenium Supplementation on Gene Expression Related to Insulin and Lipid in Infertile Polycystic Ovary Syndrome Women Candidate for In Vitro Fertilization: a Randomized, Double-Blind, Placebo-Controlled Trial. Biol Trace Elem Res 183, 218–225 (2018). https://doi.org/10.1007/s12011-017-1148-2] as concerns have been raised about integrity of the clinical trial reported here. There is also an ongoing investigation by the Iranian National Committee for Ethics in Biomedical Researches. Further editorial action will be taken as appropriate once the investigation into the concerns is complete and all parties have been given an opportunity to respond in full.

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Funding

This study was founded by a grant from the vice-chancellor for research, Shahid Beheshti, University of Medical Sciences, Tehran, Iran.

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Authors and Affiliations

  1. Infertility and Reproductive Health Research Center (IRHRC), Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Shahrzad Zadeh Modarres & Zahra Rahmati

  2. Preventative Gynecology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Zahra Heidar

  3. Department of Gynecology and Obstetrics, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran

    Fatemeh Foroozanfard

  4. Research Center for Biochemistry and Nutrition in Metabolic Diseases, Kashan University of Medical Sciences, Kashan, Iran

    Esmat Aghadavod & Zatollah Asemi

Authors
  1. Shahrzad Zadeh Modarres
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  2. Zahra Heidar
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  3. Fatemeh Foroozanfard
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  4. Zahra Rahmati
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  5. Esmat Aghadavod
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  6. Zatollah Asemi
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Contributions

ZA contributed in conception, design, statistical analysis, and drafting of the manuscript. SZ, ZH, FF, ZR, and EA contributed in data collection and manuscript drafting. All authors approved the final version for submission. ZA supervised the study.

Corresponding author

Correspondence to Zatollah Asemi.

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This study was conducted in accordance with the Declaration of Helsinki, and informed consent was obtained from all participants. The research was approved by the ethics committee of Shahid Beheshti University of Medical Sciences.

Conflict of Interest

The authors declare that they have no conflict of interest.

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Zadeh Modarres, S., Heidar, Z., Foroozanfard, F. et al. The Effects of Selenium Supplementation on Gene Expression Related to Insulin and Lipid in Infertile Polycystic Ovary Syndrome Women Candidate for In Vitro Fertilization: a Randomized, Double-Blind, Placebo-Controlled Trial. Biol Trace Elem Res 183, 218–225 (2018). https://doi.org/10.1007/s12011-017-1148-2

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