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Superoxide Dismutase Activity, Hydrogen Peroxide Steady-State Concentration, and Bactericidal and Phagocytic Activities Against Moraxella bovis, in Neutrophils Isolated from Copper-Deficient Bovines

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An Erratum to this article was published on 02 September 2016

Abstract

Copper (Cu) deficiency increases occurrence of certain infectious diseases in animals, including infectious keratoconjunctivitis in bovines, a bacterial ocular inflammation caused by Moraxella bovis. Neutrophil leukocytes constitute the first phagocytic cells to arrive at infection sites for bacterial neutralization. The objective of this work was to evaluate whether the functionality of neutrophils against M. bovis is impaired in experimentally induced Cu deficiency in bovines using high molybdenum and sulfur levels in the diet. The Cu tissue values and the periocular achromotrichia observed in +Mo animals showed that the clinic phase of Cu deficiency was reached in this group. Instead, +Cu animals have not evidenced clinical signs or biochemical parameters of hypocuprosis. On the basis of our observations, we concluded that Cu deficiency has no effect on phagocytic and bactericidal activities of neutrophils against M. bovis. However, superoxide dismutase activity and peroxide hydrogen generation were significantly different between groups. Therefore, additional research to explain these results is merited to fully characterize the consequences of Cu status on the risk for infections under field conditions.

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Compliance with Ethical Standards

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Ethics Approval

All procedures received prior review and approval by the Institutional Experimental Animal Care and Use Committee of Buenos Aires University, Faculty of Veterinary Science.

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Authors and Affiliations

  1. Cátedra de Patología, Facultad de Ciencias Veterinarias, Universidad de Buenos Aires (UBA), Chorroarín 280, C1427CWO, Buenos Aires, Argentina

    Postma Gabriela Cintia, Minatel Leonardo, Olivares Roberto Walter Israel, Schapira Andrea & Dallorso Maria Elena

  2. Cátedra de Fisicoquímica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina

    Valdez Laura Beatriz

Authors
  1. Postma Gabriela Cintia
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  2. Minatel Leonardo
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  3. Olivares Roberto Walter Israel
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  4. Schapira Andrea
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  5. Valdez Laura Beatriz
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  6. Dallorso Maria Elena
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Corresponding author

Correspondence to Postma Gabriela Cintia.

Additional information

An erratum to this article can be found at http://dx.doi.org/10.1007/s12011-016-0812-2.

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Cintia, P.G., Leonardo, M., Israel, O.R.W. et al. Superoxide Dismutase Activity, Hydrogen Peroxide Steady-State Concentration, and Bactericidal and Phagocytic Activities Against Moraxella bovis, in Neutrophils Isolated from Copper-Deficient Bovines. Biol Trace Elem Res 171, 94–100 (2016). https://doi.org/10.1007/s12011-015-0505-2

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  • DOI: https://doi.org/10.1007/s12011-015-0505-2

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