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Increased Oxidative DNA Damage in Placenta Contributes to Cadmium-Induced Preeclamptic Conditions in Rat

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Abstract

To explore the possible mechanisms of cadmium (Cd)-induced preeclamptic conditions in rats. In the present study, we introduced the in vivo model of preeclampsia by giving intraperitoneal injections of cadmium chloride (CdCl2) to pregnant rats from gestational day (GD) 4 to 19. Maternal body weights were recorded on GD 0, 14, and 20, while their systolic blood pressures (SBPs) monitored on GD 3, 11, and 18. On GD 20, rats were sacrificed and the specimens were collected. The morphological changes of placenta and kidney tissues of pregnant rats were examined by hematoxylin and eosin staining assay. Blood Cd level was detected by inductively coupled plasma mass spectrometry. Total antioxidant capacity (TAC) was evaluated using FRAP method and total nitrite (NOx) was detected with Griess reagent. Antioxidative factors and DNA damage/repair biomarkers were measured by real-time qPCR, western blot or immunohistochemistry study. The current results showed that CdCl2-treated pregnant rats developed preeclampsia (PE)-like manifestations, such as hypertension, albuminuria, with decreased TAC and increased blood Cd level, and pro-oxidative/antioxidative or DNA damage/repair biomarkers. Our study demonstrated that increased oxidative DNA damage in placenta could contribute to Cd-induced preeclamptic conditions in rat.

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Conflict of Interest

The authors declare no conflict of interests and have nothing to disclose.

This study was financially supported by the National Natural Science Foundation of China (No. 81,070,510).

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Authors and Affiliations

  1. Department of Gynecology and Obstetrics, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China

    Xiaojie Zhang, Feng Lin & Yinping Huang

  2. Department of Gynecology and Obstetrics, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China

    Zhangye Xu & Fan Wang

  3. Department of Pathophysiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

    Duyun Ye

Authors
  1. Xiaojie Zhang
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  2. Zhangye Xu
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  3. Feng Lin
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  4. Fan Wang
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  6. Yinping Huang
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Correspondence to Yinping Huang.

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Zhang, X., Xu, Z., Lin, F. et al. Increased Oxidative DNA Damage in Placenta Contributes to Cadmium-Induced Preeclamptic Conditions in Rat. Biol Trace Elem Res 170, 119–127 (2016). https://doi.org/10.1007/s12011-015-0438-9

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  • DOI: https://doi.org/10.1007/s12011-015-0438-9

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