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Zinc Chloride Inhibits Human Lens Epithelial Cell Migration and Proliferation Involved in TGF-β1 and TNF-α Signaling Pathways in HLE B-3 Cells

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Abstract

Zinc is one of the most abundant essential elements in the human body, which is an essential, coenzyme-like component of many enzymes, and is indispensable to their functions. However, high levels of zinc ions can lead to cell damage. In the present study, we explored the effects of high concentrations of zinc chloride (ZnCl2) on lens epithelial cell proliferation and migration and further investigated the effects of different concentrations of ZnCl2 on caspase-9 and caspase-12, transforming growth factor-beta 1 (TGF-β1), and tumor necrosis factor-alpha (TNF-α). We found that ZnCl2 could inhibit human lens epithelial (HLE) B-3 cell migration and induce apoptosis/necrosis. In addition, ZnCl2 can efficiently decrease the expressions of caspase-9 and caspase-12, increase the expression of TNF-α at both gene and protein levels, and thus induces cell death. Taken together, our results indicate that ZnCl2 can inhibit HLE B-3 cell migration and proliferation by decreasing the expression of TGF-β1 and increasing the expression of TNF-α and finally lead to HLE B-3 cell death.

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Acknowledgments

This work was supported by the Natural Science Foundation of Shandong province (ZR2010HM032).

Conflict of Interest

The authors declare that they have no competing interests.

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Authors and Affiliations

  1. Shandong University of Traditional Chinese Medicine, Jinan, 250002, China

    Yuxiang Du

  2. Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases of Shandong province; Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases in Universities of Shandong; Eye Institute of Shandong University of Traditional Chinese Medicine, Jinan, 250002, China

    Dadong Guo, Dongmei Liu & Hongsheng Bi

  3. Affiliated Eye Hospital of Shandong University of Traditional Chinese Medicine, Jinan, 250002, China

    Qiuxin Wu & Hongsheng Bi

Authors
  1. Yuxiang Du
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  2. Dadong Guo
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  3. Qiuxin Wu
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  4. Dongmei Liu
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  5. Hongsheng Bi
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Correspondence to Hongsheng Bi.

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Du, Y., Guo, D., Wu, Q. et al. Zinc Chloride Inhibits Human Lens Epithelial Cell Migration and Proliferation Involved in TGF-β1 and TNF-α Signaling Pathways in HLE B-3 Cells. Biol Trace Elem Res 159, 425–433 (2014). https://doi.org/10.1007/s12011-014-9979-6

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  • DOI: https://doi.org/10.1007/s12011-014-9979-6

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