Skip to main content
SpringerLink
Log in

Effects of Sodium Selenite on Aflatoxin B1-Induced Decrease of Ileal IgA+ Cell Numbers and Immunoglobulin Contents in Broilers

  • Published:
Biological Trace Element Research Aims and scope Submit manuscript

Abstract

This study was aimed to assess the protective effect of sodium selenite on the ileum mucosal immunologic injury induced by AFB1. One hundred eighty-one-day-old healthy male Avian broilers were divided into four groups of three replicates and 15 birds per replicate and fed with basal diet (control group), 0.3 mg/kg AFB1 (AFB1 group), 0.4 mg/kg Se (+Se group), and 0.3 mg/kg AFB1 + 0.4 mg/kg Se (AFB1 + Se group) respectively. The numbers of IgA+ cells of ileum were determined by immunohistochemistry as well as the contents of sIgA, IgA, IgG, and IgM in the mucosa of ileum by ELISA. Compared with those in the control group, the numbers of IgA+ cells as well as the sIgA, IgA, IgG, and IgM contents were decreased in the AFB1 group. However, compared with those in the AFB1 group, the numbers of IgA+ cells as well as the sIgA, IgA, IgG, and IgM contents were increased in the AFB1 + Se group, and these data had no difference between AFB1 + Se group and control group. It was concluded that 0.3 mg/kg AFB1 could reduce the humoral immune function of the ileum mucosa, but 0.4 mg/kg supplemented dietary selenium could protect the mucosal humoral immune function from AFB1-induced impairment.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Mohana DC, Thippeswamy S, Abhishek RU, Manjunath K (2014) Natural occurrence of Aspergillus flavus and Fusarium verticillioides, and AFB1 and FB1 contamination in maize grown in Southern Karnataka (India). Can J Plant Protect (CJPP) 2(1):17–20

    Google Scholar 

  2. Groopman JD, Kensler TW (2005) Role of metabolism and viruses in aflatoxin-induced liver cancer. Toxicol Appl Pharmacol 206(2):131–137

    Article  CAS  PubMed  Google Scholar 

  3. Rothschild L (1992) IARC classes AFB1 as class 1 human carcinogen. Food Chem News 34:62–66

    Google Scholar 

  4. Meissonnier G, Marin D, Galtier P, Bertin G, Taranu I, Oswald I, Mengheri E (2006) Modulation of the immune response by a group of fungal food contaminant, the aflatoxins. Nutrition and immunity:147–166

  5. Sur E, Celik I, Oznurlu Y, Aydin M, Oguz H, Kurtoglu V, Ozaydin T (2011) Enzyme histochemical and serological investigations on the immune system from chickens treated in ovo with aflatoxin B-1 (AFB (1)). Rev Méd Vét 162(10):443–448

    CAS  Google Scholar 

  6. Surai PF, Dvorska J (2005) Effects of mycotoxins on antioxidant status and immunity. Nottingham University, Nottingham

    Google Scholar 

  7. Celik I, Oguz H, Demet O, Donmez H, Boydak M, Sur E (2000) Efficacy of polyvinylpolypyrrolidone in reducing the immunotoxicity of aflatoxin in growing broilers. Br Poult Sci 41(4):430–439

    Article  CAS  PubMed  Google Scholar 

  8. Oguz H, Hadimli H, Kurtoglu V, Erganis O (2003) Evaluation of humoral immunity of broilers during chronic aflatoxin (50 and 100 ppb) and clinoptilolite exposure. Rev Méd Vét 154(7):483–486

    CAS  Google Scholar 

  9. Galvano F, Piva A, Ritieni A, Galvano G (2001) Dietary strategies to counteract the effects of mycotoxins: a review. J Food Prot 64(1):120–131

    CAS  PubMed  Google Scholar 

  10. Tinggi U (2003) Essentiality and toxicity of selenium and its status in Australia: a review. Toxicol Lett 137(1):103–110

    Article  CAS  PubMed  Google Scholar 

  11. Arthur JR, McKenzie RC, Beckett GJ (2003) Selenium in the immune system. J Nutr 133(5):1457S–1459S

    CAS  PubMed  Google Scholar 

  12. Peng X, H-m C, Deng J, Zuo Z, Cui W (2011) Low dietary selenium induce increased apoptotic thymic cells and alter peripheral blood T cell subsets in chicken. Biol Trace Elem Res 142(2):167–173

    Article  CAS  PubMed  Google Scholar 

  13. Peng X, Cui H, Cui Y, Deng J, Zuo Z, Fang J (2011) Lesions of thymus and decreased percentages of the peripheral blood T-cell subsets in chickens fed on diets excess in selenium. Hum Exp Toxicol 30(12):1972–1978

    Article  CAS  PubMed  Google Scholar 

  14. Gomez CO, Ferrer A, Lachmann M, Arrieta-Mendoza D, Novoa E, Roman-Bravo R (2009) Effect of Saccharomyces cerevisiae culture and selenium intake in broiler chickens exposed to low levels of aflatoxin B1 in the ration. Rev Cien, Fac Cien Vet, Univ del Zulia 19(4):390–399

    Google Scholar 

  15. Chen K, Peng X, Fang J, Cui H, Zuo Z, Deng J, Chen Z, Geng Y, Lai W, Tang L (2014) Effects of dietary selenium on histopathological changes and T cells of spleen in broilers exposed to aflatoxin B1. Int J Environ Res Public Health 11(2):1904–1913

    Article  PubMed Central  PubMed  Google Scholar 

  16. Wang F, Shu G, Peng X, Fang J, Chen K, Cui H, Chen Z, Zuo Z, Deng J, Geng Y (2013) Protective effects of sodium selenite against aflatoxin B1-induced oxidative stress and apoptosis in broiler spleen. Int J Environ Res Public Health 10(7):2834–2844

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  17. Wittig B, Zeitz M (2003) The gut as an organ of immunology. Int J Color Dis 18(3):181–187

    CAS  Google Scholar 

  18. Khan M, Akter S, Islam M, Karim M, Islam M, Kon Y (2008) The effect of selenium and vitamin E on the lymphocytes and immunoglobulin‐containing plasma cells in the lymphoid organ and mucosa‐associated lymphatic tissues of broiler chickens. Anat Histol, Embryol 37(1):52–59

    CAS  Google Scholar 

  19. Brandtzaeg P, Farstad IN, Johansen FE, Morton HC, Norderhaug IN, Yamanaka T (1999) The B‐cell system of human mucosae and exocrine glands. Immunol Rev 171(1):45–87

    Article  CAS  PubMed  Google Scholar 

  20. Kaul D, Ogra P (1997) Mucosal responses to parenteral and mucosal vaccines. Dev Biol Stand 95:141–146

    Google Scholar 

  21. Chen K, Shu G, Peng X, Fang J, Cui H, Chen J, Wang F, Chen Z, Zuo Z, Deng J, Geng Y, Lai W (2013) Protective role of sodium selenite on histopathological lesions, decreased T-cell subsets and increased apoptosis of thymus in broilers intoxicated with aflatoxin B1. Food Chem Toxicol: Int J Pub Br Ind Biol Res Assoc 59:446–454

    Article  CAS  Google Scholar 

  22. Council NR (1994) Nutrient requirements of poultry, 9th edn. National Academy, Washington, DC

    Google Scholar 

  23. Luo Q, Cui H, Peng X, Fang J, Zuo Z, Deng J, Liu J, Deng Y (2013) Intestinal IgA + Cell Numbers as well as IgA, IgG, and IgM contents correlate with mucosal humoral immunity of broilers during supplementation with high fluorine in the diets. Biol Trace Elem Res 154(1):62–72

    Article  CAS  PubMed  Google Scholar 

  24. Wu B, Cui H, Peng X, Fang J, Zuo Z, Huang J, Luo Q, Deng Y, Wang H, Liu J (2013) Changes of the serum cytokine contents in broilers fed on diets supplemented with nickel chloride. Biol Trace Elem Res 151(2):234–239

    Article  CAS  PubMed  Google Scholar 

  25. Peterson DA, McNulty NP, Guruge JL, Gordon JI (2007) IgA response to symbiotic bacteria as a mediator of gut homeostasis. Cell Host Microbe 2(5):328–339

    Article  CAS  PubMed  Google Scholar 

  26. Mantis NJ, Rol N, Corthésy B (2011) Secretory IgA's complex roles in immunity and mucosal homeostasis in the gut. Mucosal Immunol 4(6):603–611

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  27. Hanly WC, Artwohl JE, Bennett BT (1995) Review of polyclonal antibody production procedures in mammals and poultry. ILAR J 37(3):93–118

    Article  PubMed  Google Scholar 

  28. Cerutti A, Rescigno M (2008) The biology of intestinal immunoglobulin A responses. Immunity 28(6):740–750

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  29. Ratcliffe MJ (2006) Antibodies, immunoglobulin genes and the bursa of Fabricius in chicken B cell development. Dev Comp Immunol 30(1):101–118

    Article  CAS  PubMed  Google Scholar 

  30. Boes M (2000) Role of natural and immune IgM antibodies in immune responses. Mol Immunol 37(18):1141–1149

    Article  CAS  PubMed  Google Scholar 

  31. Jolles S, Kaveri S, Orange J (2009) Current understanding and future directions. Clin Exp Immunol 158(s1):68–70

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  32. Perdigon G, Alvarez S, Rachid M, Agüero G, Gobbato N (1995) Immune system stimulation by probiotics. J Dairy Sci 78(7):1597–1606

    Article  CAS  PubMed  Google Scholar 

  33. Hussan M, Khan M, Lucky N (2009) Immunohistochemical study of the postnatal development of lymphoid tissues and mucosa of broilers. Bangladesh J Vet Med 7(1):253–258

    Google Scholar 

  34. Ghosh R, Chauhan H, Roy S (1990) Immunosuppression in broilers under experimental aflatoxicosis. Br Vet J 146(5):457–462

    Article  CAS  PubMed  Google Scholar 

  35. Wan X, Yang Z, Yang W, Jiang S, Zhang G, Johnston S, Chi F (2013) Toxicity of increasing aflatoxin B1 concentrations from contaminated corn with or without clay adsorbent supplementation in ducklings. Poult Sci 92(5):1244–1253

    Article  CAS  PubMed  Google Scholar 

  36. Sur E, Celik I (2003) Effects of aflatoxin B1 on the development of the bursa of Fabricius and blood lymphocyte acid phosphatase of the chicken. Br Poult Sci 44(4):558–566

    Article  CAS  PubMed  Google Scholar 

  37. Ortatatli M, Oğuz H, Hatipoğlu F, Karaman M (2005) Evaluation of pathological changes in broilers during chronic aflatoxin (50 and 100 ppb) and clinoptilolite exposure. Res Vet Sci 78(1):61–68

    Article  CAS  PubMed  Google Scholar 

  38. Khatke PA, Singh R, Mandal A, Tyagi PK (2013) Ability of biological adsorbents to ameliorate aflatoxicosis in broiler chicken: effect on blood biochemicals and organ weights. Indian J Poult Sci 48(1):117–121

    Google Scholar 

  39. Mowat AM (2003) Anatomical basis of tolerance and immunity to intestinal antigens. Nat Rev Immunol 3(4):331–341

    Article  CAS  PubMed  Google Scholar 

  40. Shang H-F, Wang Y-Y, Lai Y-N, Chiu W-C, Yeh S-L (2004) Effects of arginine supplementation on mucosal immunity in rats with septic peritonitis. Clin Nutr 23(4):561–569

    Article  CAS  PubMed  Google Scholar 

  41. Chen K, Yuan S, Chen J, Peng X, Wang F, Cui H, Fang J (2013) Effects of sodium selenite on the decreased percentage of T cell subsets, contents of serum IL-2 and IFN-γ induced by aflatoxin B1 in broilers. Res Vet Sci 95(1):143–145

    Article  CAS  PubMed  Google Scholar 

  42. He Y, Fang J, Peng X, Cui H, Zuo Z, Deng J, Chen Z, Lai W, Shu G, Tang L (2014) Effects of sodium selenite on aflatoxin B1-induced decrease of ileac T-cell and the mRNA contents of IL-2, IL-6 and TNF-α in broilers. Biol Trace Elem Res. doi:10.1007/s12011-014-9999-2

    Google Scholar 

  43. Surai PF (2002) Natural antioxidants in avian nutrition and reproduction. Nottingham University, Nottingham

    Google Scholar 

  44. Corrier D (1991) Mycotoxicosis: mechanisms of immunosuppression. Vet Immunol Immunopathol 30(1):73–87

    Article  CAS  PubMed  Google Scholar 

  45. Shin Y, Blackwood JM, Bae I-T, Arey BW, Exarhos GJ (2007) Synthesis and stabilization of selenium nanoparticles on cellulose nanocrystal. Mater Lett 61(21):4297–4300

    Article  CAS  Google Scholar 

  46. Steinbrenner H, Sies H (2009) Protection against reactive oxygen species by selenoproteins. Biochim Biophys Acta (BBA)-Gen Subj 1790(11):1478–1485

    Article  CAS  Google Scholar 

  47. Surai P (2002) Selenium in poultry nutrition 1. Antioxidant properties, deficiency and toxicity. World's Poult Sci J 58(03):333–347

    Article  Google Scholar 

Download references

Acknowledgments

This work was supported by the program for Changjiang scholars and university innovative research team (IRT 0848) and the Science and Technology Department of Sichuan Province (2012FZ0066).

Conflict of Interest

The authors have no financial and non-financial conflict of interest.

Author information

Authors and Affiliations

  1. Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya’an, 625014, China

    Yang He, Jing Fang, Xi Peng, Hengmin Cui, Zhicai Zuo, Junliang Deng, Zhengli Chen, Yi Geng, Weimin Lai, Gang Shu & Li Tang

Authors
  1. Yang He
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jing Fang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xi Peng
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hengmin Cui
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Zhicai Zuo
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Junliang Deng
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Zhengli Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Yi Geng
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Weimin Lai
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Gang Shu
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Li Tang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Xi Peng or Hengmin Cui.

Additional information

Yang He and Jing Fang contributed equally in this study.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

He, Y., Fang, J., Peng, X. et al. Effects of Sodium Selenite on Aflatoxin B1-Induced Decrease of Ileal IgA+ Cell Numbers and Immunoglobulin Contents in Broilers. Biol Trace Elem Res 160, 49–55 (2014). https://doi.org/10.1007/s12011-014-0035-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12011-014-0035-3

Keywords

Search

Navigation