Abstract
Fluoride compounds are abundant and widely distributed in the environment at a variety of concentrations. Further, fluoride induces toxic effects in target organs such as the liver and kidney. In this study, we performed an early analysis of renal function using a clearance technique in Wistar rats acutely exposed to fluoride at a plasma concentration of 0.625 μg/ml. Our results revealed that fluoride, at a concentration close to the concentration present in the serum after environmental exposure, induced a significant tubular dysfunction, resulting in diluted urine, impaired protein reabsorption, and increased calcium and phosphate urinary excretion. Our work demonstrates that even acute exposures to low concentrations of NaF may induce renal damage and confirms that, after exposure, the kidney participates directly in the calcium and phosphate deficiencies observed in fluoride-exposed populations.
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References
Monsour PA, Harbrow DJ, Warshawsky H (1989) Effects of acute doses of sodium fluoride on the morphology and the detectable calcium associated with secretory ameloblasts in rat incisors. J Histochem Cytochem 37(4):463–471
Dissanayake C (1991) The fluoride problem in the ground water of Sri Lanka—environmental management and health. Int J Environ Stud 38(2–3):137–155
Dote T, Kono K, Usuda K, Nishiura H, Tagawa T, Miyata K, Shimahara M, Hashiguchi N, Senda J, Tanaka Y (2000) Toxicokinetics of intravenous fluoride in rats with renal damage caused by high-dose fluoride exposure. Int Arch Occup Environ Heal 73 Suppl:S90–92
Dissanayake CB, Chandrajith R (2007) Medical geology in tropical countries with special reference to Sri Lanka. Environ Geochem Heal 29(2):155–162. doi:10.1007/s10653-006-9070-0
Shashi ATS (2000) Histopathology of fluoride-induced hepatotoxicity in rabbits. Fluoride 34:34–42
Usuda K, Kono K, Dote T, Nishiura K, Miyata K, Nishiura H, Shimahara M, Sugimoto K (1998) Urinary biomarkers monitoring for experimental fluoride nephrotoxicity. Arch Toxicol 72(2):104–109
Xiong X, Liu J, He W, Xia T, He P, Chen X, Yang K, Wang A (2007) Dose–effect relationship between drinking water fluoride levels and damage to liver and kidney functions in children. Environ Res 103(1):112–116. doi:10.1016/j.envres.2006.05.008
Borke JL, Whitford GM (1999) Chronic fluoride ingestion decreases 45Ca uptake by rat kidney membranes. J Nutr 129(6):1209–1213
Tiwari S, Gupta SK, Kumar K, Trivedi R, Godbole MM (2004) Simultaneous exposure of excess fluoride and calcium deficiency alters VDR, CaR, and calbindin D 9 k mRNA levels in rat duodenal mucosa. Calcif Tissue Int 75(4):313–320. doi:10.1007/s00223-004-0225-7
Barbier O, Arreola-Mendoza L, Del Razo LM (2010) Molecular mechanisms of fluoride toxicity. Chem Biol Interact 188(2):319–333. doi:10.1016/j.cbi.2010.07.011
Chandrajith R, Nanayakkara S, Itai K, Aturaliya TN, Dissanayake CB, Abeysekera T, Harada K, Watanabe T, Koizumi A (2011) Chronic kidney diseases of uncertain etiology (CKDue) in Sri Lanka: geographic distribution and environmental implications. Environ Geochem Heal 33(3):267–278. doi:10.1007/s10653-010-9339-1
Harvey AM, Malvin RL (1966) The effect of androgenic hormones on creatinine secretion in the rat. J Physiol 184(4):883–888
Willinger CC, Moschen I, Kulmer S, Pfaller W (1995) The effect of sodium fluoride at prophylactic and toxic doses on renal structure and function in the isolated perfused rat kidney. Toxicology 95(1–3):55–71
Kobayashi CA, Leite AL, Silva TL, Santos LD, Nogueira FC, Oliveira RC, Palma MS, Domont GB, Buzalaf MA (2009) Proteomic analysis of kidney in rats chronically exposed to fluoride. Chem Biol Interact 180(2):305–311. doi:10.1016/j.cbi.2009.03.009
Suketa Y, Mikami E (1977) Changes in urinary ion excretion and related renal enzyme activities in fluoride-treated rats. Toxicol Appl Pharmacol 40(3):551–559
Mazze RI (1976) Methoxyflurane nephropathy. Environ Heal Perspect 15:111–119
Roman RJ, Carter JR, North WC, Kauker ML (1977) Renal tubular site of action of fluoride in Fischer 344 rats. Anesthesiology 46(4):260–264
Mitsui G, Dote T, Yamadori E, Imanishi M, Nakayama S, Ohnishi K, Kono K (2010) Toxicokinetics and metabolism deteriorated by acute nephrotoxicity after a single intravenous injection of hydrofluoric acid in rats. J Occup Heal 52(6):395–399
Imanishi M, Dote T, Tsuji H, Tanida E, Yamadori E, Kono K (2009) Time-dependent changes of blood parameters and fluoride kinetics in rats after acute exposure to subtoxic hydrofluoric acid. J Occup Heal 51(4):287–293
Nabavi SF, Habtemariam S, Jafari M, Sureda A, Nabavi SM (2012) Protective role of gallic acid on sodium fluoride induced oxidative stress in rat brain. Bull Environ Contam Toxicol 89(1):73–77. doi:10.1007/s00128-012-0645-4
Nabavi SF, Moghaddam AH, Eslami S, Nabavi SM (2012) Protective effects of curcumin against sodium fluoride-induced toxicity in rat kidneys. Biol Trace Elem Res 145(3):369–374. doi:10.1007/s12011-011-9194-7
Acknowledgments
This research was supported by the Consejo Nacional de Ciencia y Tecnología (CONACyT, grant 152416) and the Instituto Politécnico Nacional (IPN/SIP grant 20082576), México. M.P. Santoyo-Sanchez was awarded a fellowship from CONACyT (grant 28849).
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Santoyo-Sanchez, M.P., del Carmen Silva-Lucero, M., Arreola-Mendoza, L. et al. Effects of Acute Sodium Fluoride Exposure on Kidney Function, Water Homeostasis, and Renal Handling of Calcium and Inorganic Phosphate. Biol Trace Elem Res 152, 367–372 (2013). https://doi.org/10.1007/s12011-013-9622-y
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DOI: https://doi.org/10.1007/s12011-013-9622-y