Skip to main content
SpringerLink
Log in

Possible Correlation between Selenoprotein W and Myogenic Regulatory Factors in Chicken Embryonic Myoblasts

  • Published:
Biological Trace Element Research Aims and scope Submit manuscript

Abstract

The biological function of selenium (Se) is mainly elicited through Se-containing proteins. Selenoprotein W (SelW), one member of the selenoprotein family, is essential for the normal function of the skeletal muscle system. To investigate the possible relationship of Se in the process of differentiation in chicken myoblasts and the expression of SelW, the cultured chicken embryonic myoblasts were incubated with sodium selenite at different concentrations for 72 h, and then the mRNA levels of SelW and myogenic regulatory factors (MRFs) in myoblasts were determined at 12, 24, 48, and 72 h, respectively. Furthermore, the correlation between SelW mRNA expression and MRF mRNA expression was assessed. The results showed that the sodium selenite medium enhanced the mRNA expression of SelW, Myf-5, MRF4, and myogenin in chicken myoblasts. The mRNA expression levels of MRFs were significantly correlated with those of SelW at 24, 48, and 72 h. These data demonstrate that Se is involved in the differentiation of chicken embryonic myoblasts, and SelW showed correlation with MRFs.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Combs GF Jr, Clark LC, Turnbull BW (2001) An analysis of cancer prevention by selenium. Biofactors 14:153–159

    Article  PubMed  CAS  Google Scholar 

  2. Li JL, Gao R, Li S, Wang JT, Tang ZX, Xu SW (2010) Testicular toxicity induced by dietary cadmium in cocks and ameliorative effect by selenium. Biometals 23:695–705

    Article  PubMed  CAS  Google Scholar 

  3. Schweizer U, Schomburg L, Savaskan NE (2004) The neurobiology of selenium: lessons from transgenic mice. J Nutr 134:707–710

    PubMed  CAS  Google Scholar 

  4. Rayman MP (2000) The importance of selenium to human health. Lancet 356:233–241

    Article  PubMed  CAS  Google Scholar 

  5. Hoffmann PR, Berry MJ (2008) The influence of selenium on immune responses. Mol Nutr Food Res 52:1273–1280

    Article  PubMed  CAS  Google Scholar 

  6. Kaur P, Bansal MP (2005) Effect of selenium-induced oxidative stress on the cell kinetics in testis and reproductive ability of male mice. Nutrition 21:351–357

    Article  PubMed  CAS  Google Scholar 

  7. Martin-Romero FJ, Kryukov GV, Lobanov AV, Carlson BA, Lee BJ, Gladyshev VN, Hatfield DL (2001) Selenium metabolism in Drosophila: selenoproteins, selenoprotein mRNA expression, fertility, and mortality. J Biol Chem 276:29798–29804

    Article  PubMed  CAS  Google Scholar 

  8. Jackson-Rosario SE, Self WT (2010) Targeting selenium metabolism and selenoproteins: novel avenues for drug discovery. Metallomics 2:112–116

    Article  PubMed  CAS  Google Scholar 

  9. Chariot P, Bignani O (2003) Skeletal muscle disorders associated with selenium deficiency in humans. Muscle Nerve 27:662–668

    Article  PubMed  CAS  Google Scholar 

  10. Mahmoud KZ, Edens FW (2005) Influence of organic selenium on hsp70 response of heat-stressed and enteropathogenic Escherichia coli-challenged broiler chickens (Gallus gallus). Comp Biochem Physiol C Toxicol Pharmacol 141:69–75

    Article  PubMed  Google Scholar 

  11. Schubert JR, Muth OH, Oldfield JE, Remmert LF (1961) Experimental results with selenium in white muscle disease of lambs and calves. Fed Proc 20:689–694

    PubMed  CAS  Google Scholar 

  12. Van Vleet JF, Ferrans VJ (1976) Ultrastructural changes in skeletal muscle of selenium-vitamin E-deficient chicks. Am J Vet Res 37:1081–1089

    PubMed  Google Scholar 

  13. Bartholomew A, Latshaw D, Swayne DE (1998) Changes in blood chemistry, hematology, and histology caused by a selenium/vitamin E deficiency and recovery in chicks. Biol Trace Elem Res 62:7–16

    Article  PubMed  CAS  Google Scholar 

  14. Ou BR, Jiang MJ, Lin CH, Liang YC, Lee KJ, Yeh JY (2011) Characterization and expression of chicken selenoprotein W. Biometals 24:323–333

    Article  PubMed  CAS  Google Scholar 

  15. Gu J, Royland JE, Wiggins RC, Konat GW (1997) Selenium is required for normal upregulation of myelin genes in differentiating oligodendrocytes. J Neurosci Res 47:626–635

    Article  PubMed  CAS  Google Scholar 

  16. Nishina A, Sekiguchi A, Fukumoto RH, Koketsu M, Furukawa S (2007) Selenazoles (selenium compounds) facilitate survival of cultured rat pheochromocytoma PC12 cells after serum-deprivation and stimulate their neuronal differentiation via activation of Akt and mitogen-activated protein kinase, respectively. Biochem Biophys Res Commun 352:360–365

    Article  PubMed  CAS  Google Scholar 

  17. Asakura A, Seale P, Girgis-Gabardo A, Rudnicki MA (2002) Myogenic specification of side population cells in skeletal muscle. J Cell Biol 159:123–134

    Article  PubMed  CAS  Google Scholar 

  18. Muntoni F, Brown S, Sewry C, Patel K (2002) Muscle development genes: their relevance in neuromuscular disorders. Neuromuscul Disord 12:438–446

    Article  PubMed  Google Scholar 

  19. Charge SB, Rudnicki MA (2004) Cellular and molecular regulation of muscle regeneration. Physiol Rev 84:209–238

    Article  PubMed  CAS  Google Scholar 

  20. Tajbakhsh S, Buckingham M (2000) The birth of muscle progenitor cells in the mouse: spatiotemporal considerations. Curr Top Dev Biol 48:225–268

    Article  PubMed  CAS  Google Scholar 

  21. Weintraub H, Davis R, Tapscott S, Thayer M, Krause M, Benezra R, Blackwell TK, Turner D, Rupp R, Hollenberg S et al (1991) The myoD gene family: nodal point during specification of the muscle cell lineage. Science 251:761–766

    Article  PubMed  CAS  Google Scholar 

  22. Gayraud-Morel B, Chretien F, Flamant P, Gomes D, Zammit PS, Tajbakhsh S (2007) A role for the myogenic determination gene Myf5 in adult regenerative myogenesis. Dev Biol 312:13–28

    Article  PubMed  CAS  Google Scholar 

  23. Behne D, Kyriakopoulos A (2001) Mammalian selenium-containing proteins. Annu Rev Nutr 21:453–473

    Article  PubMed  CAS  Google Scholar 

  24. Kryukov GV, Gladyshev VN (2004) The prokaryotic selenoproteome. EMBO Rep 5:538–543

    Article  PubMed  CAS  Google Scholar 

  25. Zhang Y, Fomenko DE, Gladyshev VN (2005) The microbial selenoproteome of the Sargasso Sea. Genome Biol 6:R37

    Article  PubMed  Google Scholar 

  26. Papp LV, Lu J, Holmgren A, Khanna KK (2007) From selenium to selenoproteins: synthesis, identity, and their role in human health. Antioxid Redox Signal 9:775–806

    Article  PubMed  CAS  Google Scholar 

  27. Li JL, Ruan HF, Li HX, Li S, Xu SW, Tang ZX (2011) Molecular cloning, characterization and mRNA expression analysis of a novel selenoprotein: avian selenoprotein W from chicken. Mol Biol Rep 38:4015–4022

    Article  PubMed  CAS  Google Scholar 

  28. Loflin J, Lopez N, Whanger PD, Kioussi C (2006) Selenoprotein W during development and oxidative stress. J Inorg Biochem 100:1679–1684

    Article  PubMed  CAS  Google Scholar 

  29. Ruan H, Zhang Z, Wu Q, Yao H, Li J, Li S, Xu S (2012) Selenium regulates gene expression of selenoprotein W in chicken skeletal muscle system. Biol Trace Elem Res 145:59–65

    Article  PubMed  CAS  Google Scholar 

  30. Noh OJ, Park YH, Chung YW, Kim IY (2010) Transcriptional regulation of selenoprotein W by MyoD during early skeletal muscle differentiation. J Biol Chem 285:40496–40507

    Article  PubMed  CAS  Google Scholar 

  31. Peirson SN, Butler JN, Foster RG (2003) Experimental validation of novel and conventional approaches to quantitative real-time PCR data analysis. Nucleic Acids Res 31:e73

    Article  PubMed  Google Scholar 

  32. Pfaffl MW (2001) A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29:e45

    Article  PubMed  CAS  Google Scholar 

  33. Avanzo JL, de Mendonca CX Jr, Pugine SM, de Cerqueira Cesar M (2001) Effect of vitamin E and selenium on resistance to oxidative stress in chicken superficial pectoralis muscle. Comp Biochem Physiol C Toxicol Pharmacol 129:163–173

    Article  PubMed  CAS  Google Scholar 

  34. Hassan S, Hakkarainen J, Jonsson L, Tyopponen J (1990) Histopathological and biochemical changes associated with selenium and vitamin E deficiency in chicks. Zentralbl Veterinarmed A 37:708–720

    PubMed  CAS  Google Scholar 

  35. Hoffmann FW, Hashimoto AC, Shafer LA, Dow S, Berry MJ, Hoffmann PR (2010) Dietary selenium modulates activation and differentiation of CD4+ T cells in mice through a mechanism involving cellular free thiols. J Nutr 140:1155–1161

    Article  PubMed  CAS  Google Scholar 

  36. Perry RL, Rudnick MA (2000) Molecular mechanisms regulating myogenic determination and differentiation. Front Biosci 5:D750–767

    Article  PubMed  CAS  Google Scholar 

  37. Salmon M, Owens GK, Zehner ZE (2009) Over-expression of the transcription factor, ZBP-89, leads to enhancement of the C2C12 myogenic program. Biochim Biophys Acta 1793:1144–1155

    Article  PubMed  CAS  Google Scholar 

  38. Patapoutian A, Yoon JK, Miner JH, Wang S, Stark K, Wold B (1995) Disruption of the mouse MRF4 gene identifies multiple waves of myogenesis in the myotome. Development 121:3347–3358

    PubMed  CAS  Google Scholar 

  39. Rawls A, Morris JH, Rudnicki M, Braun T, Arnold HH, Klein WH, Olson EN (1995) Myogenin's functions do not overlap with those of MyoD or Myf-5 during mouse embryogenesis. Dev Biol 172:37–50

    Article  PubMed  CAS  Google Scholar 

  40. Zhang W, Behringer RR, Olson EN (1995) Inactivation of the myogenic bHLH gene MRF4 results in up-regulation of myogenin and rib anomalies. Genes Dev 9:1388–1399

    Article  PubMed  CAS  Google Scholar 

  41. Yoon JK, Olson EN, Arnold HH, Wold BJ (1997) Different MRF4 knockout alleles differentially disrupt Myf-5 expression: cis-regulatory interactions at the MRF4/Myf-5 locus. Dev Biol 188:349–362

    Article  PubMed  CAS  Google Scholar 

  42. Yeh JY, Beilstein MA, Andrews JS, Whanger PD (1995) Tissue distribution and influence of selenium status on levels of selenoprotein W. FASEB J 9:392–396

    PubMed  CAS  Google Scholar 

  43. Jeong DW, Kim EH, Kim TS, Chung YW, Kim H, Kim IY (2004) Different distributions of selenoprotein W and thioredoxin during postnatal brain development and embryogenesis. Mol Cells 17:156–159

    PubMed  CAS  Google Scholar 

  44. Whanger PD (2000) Selenoprotein W: a review. Cell Mol Life Sci 57:1846–1852

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This study was supported by the National Natural Science Foundation of China (30871902), the Science Foundation of the Education Department of Heilongjiang Province (11551030), the Postdoctoral Science Foundation (grant no. LRB06-262), the Postdoctoral Science Foundation of Heilongjiang Province (grant no. LBH-Z07250), and the Study Abroad Foundation of Heilongjiang Province (LC201031). The authors thank the members in the veterinary internal medicine laboratory in the College of Veterinary Medicine, Northeast Agricultural University, for help in analyzing the data.

Author information

Authors and Affiliations

  1. Department of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People’s Republic of China

    Qiong Wu, Hai-Dong Yao, Zi-Wei Zhang, Bo Zhang, Fan-Yu Meng & Shi-Wen Xu

  2. Wildlife Resource College, Northeast Forestry University, Ministry of Education, Harbin, People’s Republic of China

    Xiao-Long Wang

  3. Center of Conservation Medicine and Ecological Safety, Northeast Forestry University, Ministry of Education, Harbin, People’s Republic of China

    Xiao-Long Wang

Authors
  1. Qiong Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hai-Dong Yao
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Zi-Wei Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Bo Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Fan-Yu Meng
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Shi-Wen Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Xiao-Long Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Shi-Wen Xu or Xiao-Long Wang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wu, Q., Yao, HD., Zhang, ZW. et al. Possible Correlation between Selenoprotein W and Myogenic Regulatory Factors in Chicken Embryonic Myoblasts. Biol Trace Elem Res 150, 166–172 (2012). https://doi.org/10.1007/s12011-012-9520-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12011-012-9520-8

Keywords

Search

Navigation