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Chromium, Selenium, and Zinc Multimineral Enriched Yeast Supplementation Ameliorates Diabetes Symptom in Streptozocin-Induced Mice

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Abstract

Chromium, selenium, and zinc malnutrition has been implicated in the pathogenesis of diabetic mellitus. This study aims to investigate the effects of novel multiminerals-enriched yeast (MMEY) which are minerals supplementation containing elevated levels of chromium, selenium, and zinc simultaneously in a diabetic animal model. Streptozocin-induced diabetic male Balb/c mice (n = 80) were randomly divided into diabetes control group and three treatment groups. They were administrated oral gavages with low, medium, or high doses of MMEY, respectively. Meanwhile, healthy male Balb/c mice (n = 40) of the same body weight were randomly assigned into normal control group and high dose of MMEY control group. After 8 weeks duration of treatment, the animals were sacrificed by cervical dislocation. Serum glucose concentrations, lipid profiles, oxidative/antioxidant, and immunity status were determined. No significant adverse effects were observed in the high-dose MMEY control group. Treatment of the diabetic mice with medium- or high-dose MMEY significantly decreased serum glucose, triglyceride, total cholesterol, and malondialdehyde and increased high-density lipoprotein cholesterol, glutathione, and the activities of superoxide dismutase and glutathione peroxidase. In addition, MMEY ameliorated the pathological damage of the pancreatic islets, elevated the thymus or spleen coefficient, and increased the expressions of interleukin-2 and -4 in spleen lymphocytes compared with unsupplemented diabetic mice. In conclusion, these results indicate that supplemental MMEY inhibits hyperglycemia, abates oxidative stress, modulates disorders of lipid metabolism, and reduces the impairment of immune function in diabetic mice; especially notable are the protective effects of medium doses of MMEY on the islet cells of diabetic mice.

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Abbreviations

MMEY:

multiminerals-enriched yeast

Cr:

chromium

Se:

selenium

Zn:

zinc

STZ:

streptozocin

BSA:

bovine serum albumin

GLU:

glucose

TG:

triacylglycerol

TC:

total cholesterol

HDL:

high-density lipoprotein

MDA:

malondialdehyde

SOD:

superoxide dismutase

GSH:

glutathione

GPx:

glutathione peroxidase

IL-2:

interleukin-2

IL-4:

interleukin-4

TBARS:

thiobarbituric acid reactive substances

SD:

standard deviation

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Acknowledgments

We are grateful to Fangfang Song, Biyun Zhang, Dr. Di Wang, Shuang Rong, Wei Yang, and Ping Yao (Huazhong University of Science and Technology) for their support and guidance. The National Natural Science Foundation of China (NSFC-30872116) and the National High Technology Research and Development Program of China (863 Program, 2010AA023005) supported this study.

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Authors and Affiliations

  1. Department of Nutrition and Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan, 430030, People’s Republic of China

    Jun Liu, Wei Bao, Man Jiang, Yan Zhang, Xiping Zhang & Liegang Liu

  2. College of Life Science and Technology, Xinjiang University, Urumqi, Xinjiang, 830046, People’s Republic of China

    Jun Liu

  3. MOE Key Laboratory of Environment and Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, People’s Republic of China

    Wei Bao, Yan Zhang, Xiping Zhang & Liegang Liu

  4. Huai’an Center for Disease Control and Prevention, 118 North Huaihai Road, Huai’an, 223001, People’s Republic of China

    Man Jiang

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  1. Jun Liu
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  2. Wei Bao
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  3. Man Jiang
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  4. Yan Zhang
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  6. Liegang Liu
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Correspondence to Liegang Liu.

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Liu, J., Bao, W., Jiang, M. et al. Chromium, Selenium, and Zinc Multimineral Enriched Yeast Supplementation Ameliorates Diabetes Symptom in Streptozocin-Induced Mice. Biol Trace Elem Res 146, 236–245 (2012). https://doi.org/10.1007/s12011-011-9248-x

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