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Effects of Extremely Low-Frequency Magnetic Field on Caspase Activities and Oxidative Stress Values in Rat Brain

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Abstract

This study was aimed to investigate the effect of extremely low-frequency magnetic field (ELF-MF) on apoptosis and oxidative stress values in the brain of rat. Rats were exposed to 100 and 500 µT ELF-MF, which are the safety standards of public and occupational exposure for 2 h/day for 10 months. Brain tissues were immunohistochemically stained for the active (cleaved) caspase-3 in order to measure the apoptotic index by a semi-quantitative scoring system. In addition, the levels of catalase (CAT), malondialdehyde (MDA), myeloperoxidase (MPO), total antioxidative capacity (TAC), total oxidant status (TOS), and oxidative stress index (OSI) were measured in rat brain. Final score of apoptosis and MPO activity were not significantly different between the groups. CAT activity decreased in both exposure groups (p < 0.05), while TAC was found to be lower in ELF 500 group than those in ELF-100 and sham groups (p < 0.05). MDA, TOS, and OSI values were found to be higher in ELF-500 group than those in ELF-100 and sham groups (p < 0.05). In conclusion, apoptosis was not changed by long-term ELF-MF exposure, while both 100 and 500 µT ELF-MF exposure induced toxic effect in the rat brain by increasing oxidative stress and diminishing antioxidant defense system.

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Abbreviations

ELF-MF:

Extremely low-frequency magnetic field

CAT:

Catalase

MDA:

Malondialdehyde

MPO:

Myeloperoxidase

TAC:

Total antioxidative capacity

TOS:

Total oxidant status

OSI:

Oxidative stress index

ROS:

Reactive oxygen substances

LPO:

Lipid peroxidation

TBA:

Thiobarbituric acid

ICNIRP:

International Commission on Non Ionizing Radiation Protection

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Authors and Affiliations

  1. Department of Biophysics, Medical School of Dicle University, 21280, Diyarbakır, Turkey

    Mehmet Zulkuf Akdag & Suleyman Dasdag

  2. Department of Biochemistry, Medical School of Uludag University, Bursa, Turkey

    Engin Ulukaya

  3. Department of Pathology, Medical School of Duzce University, Duzce, Turkey

    Ali Kemal Uzunlar

  4. Department of Anatomy, Medical School of Uludag University, Bursa, Turkey

    Mustafa Ayberk Kurt

  5. Department of Biochemistry, Medical School of Harran University, Urfa, Turkey

    Abdullah Taşkın

Authors
  1. Mehmet Zulkuf Akdag
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  2. Suleyman Dasdag
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  3. Engin Ulukaya
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  4. Ali Kemal Uzunlar
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  5. Mustafa Ayberk Kurt
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  6. Abdullah Taşkın
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Correspondence to Suleyman Dasdag.

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Akdag, M.Z., Dasdag, S., Ulukaya, E. et al. Effects of Extremely Low-Frequency Magnetic Field on Caspase Activities and Oxidative Stress Values in Rat Brain. Biol Trace Elem Res 138, 238–249 (2010). https://doi.org/10.1007/s12011-010-8615-3

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