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Trace Elements and Metallothionein in Liver and Kidney of Felis catus

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Abstract

Trace metals such as Zn, Cu, and Fe are essential for life; differently, no biochemical function is known for Cd. Changes in dietary metal concentrations can cause deficiency or toxicity. Studies on trace elements in cat are lacking. This paper aimed to analyze Zn, Cu, Fe and Cd concentrations in liver and kidney of pathological domestic cat and to isolate metallothionein (MT) in these tissues. It was not possible to explore a possible correlation between metal concentrations and pathologies because the incidence for each of them was too low. Fe was the most abundant metal; in particular, the liver accumulates average Fe concentrations one order of magnitude higher than Zn and Cu, ranging from 66.75 and 1,444.23 µg/g. Significantly, higher levels of Fe were found in the liver of elder animals. Zn concentrations varied between 26.31 and 84.78 µg/g in the liver whereas in the kidney, ranged between 7.69 and 71.15 µg/g. Cu concentrations were between 2.37 and 112.91 µg/g in liver and between 2.12 and 9.85 µg/g in kidney. Cd was the least abundant metal with the exception of the kidney of the oldest cats where it reached a maximum of 13.71 µg/g. Gel-filtration metal distribution profiles from cytosolic extracts revealed the presence of Cd, Cu, Zn thioneins either in the liver or in the kidney. Because tissue samples were taken from pathological cats from different breed and age, care must be taken to use these data as a baseline profile of trace elements in healthy animals. Our results are indicative that for some specimens the feed levels of Fe and Cu could be higher than the optimal dietary intake and in few cats, there was also an exposure to Cd that was counteracted by MT biosynthesis.

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Acknowledgments

The authors thank Prof. Giuliano Bettini (Dipartimento di Sanità Pubblica Veterinaria e Patologia Animale) for providing some of the biological samples analyzed in the present paper. This work was supported by RFO grant (Alma Mater Studiorum, University of Bologna).

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Authors and Affiliations

  1. Veterinary Clinical Department, Alma Mater Studiorum, University of Bologna, Via Tolara di Sopra 50, 40064, Ozzano Emilia, Bologna, Italy

    Giulia Andreani, Stefano Cottignoli, Bruno Perfetti, Emilio Carpenè & Gloria Isani

  2. Department of Biochemistry, Veterinary Medicine Faculty, Ankara University, Ankara, Turkey

    Gorkem Kismali

Authors
  1. Giulia Andreani
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  2. Stefano Cottignoli
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  3. Bruno Perfetti
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  4. Gorkem Kismali
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  5. Emilio Carpenè
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  6. Gloria Isani
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Correspondence to Gloria Isani.

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Andreani, G., Cottignoli, S., Perfetti, B. et al. Trace Elements and Metallothionein in Liver and Kidney of Felis catus . Biol Trace Elem Res 137, 177–189 (2010). https://doi.org/10.1007/s12011-009-8569-5

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  • DOI: https://doi.org/10.1007/s12011-009-8569-5

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