Abstract
The present study was conducted to investigate the effects of chromium histidinate (CrHis) against experimentally induced type II diabetes and on chromium (Cr), zinc (Zn), selenium (Se), manganese (Mn), iron (Fe), and copper (Cu) in serum, liver, and kidney of diabetic rats. The male Wistar rats (n = 60, 8 weeks old) were divided into four groups. Group I received a standard diet (12% of calories as fat); group II were fed standard diet and received CrHis (110 mcg CrHis/kg body weight per day); group III received a high-fat diet (HFD; 40% of calories as fat) for 2 weeks and then were injected with streptozotocin (STZ) on day 14 (STZ, 40 mg/kg i.p.; HFD/STZ); group IV were treated as group III (HFD/STZ) but supplemented with 110 mcg CrHis/kg body weight per day. The mineral concentrations in the serum and tissue were determined by atomic absorption spectrometry. Compared to the HFD/STZ group, CrHis significantly increased body weight and reduced blood glucose in diabetic rats (p < 0.001). Concentrations of Cr, Zn, Se, and Mn in serum, liver, and kidney of the diabetic rats were significantly lower than in the control rats (p < 0.0001). In contrast, higher Fe and Cu levels were found in serum and tissues from diabetic versus the non-diabetic rats (p < 0.001). Chromium histidinate supplementation increased serum, liver, and kidney concentrations of Cr and Zn both in diabetic and non-diabetic rats (p < 0.001). Chromium supplementation increased Mn and Se levels in diabetic rats (p < 0.001); however, it decreased Cu levels in STZ-treated group (p < 0.001). Chromium histidinate supplementation did not affect Fe levels in both groups (p > 0.05). The results of the present study conclude that supplementing Cr to the diet of diabetic rats influences serum and tissue Cr, Zn, Se, Mn, and Cu concentrations.
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References
Broadhurst CL, Domenico P (2006) Clinical studies on chromium picolinate supplementation in diabetes mellitus—a review. Diabetes Technol Ther 86:677–687
Zargar AH, Shah NA, Massodi SR (1998) Copper, zinc and magnesium levels in non insulin-dependent diabetes mellitus. Postgrad Med J 74:665–668
Barnard RJ, Roberts CK, Varon SM, Berger JJ (1998) Diet-induced insulin resistance precedes other aspects of the metabolic syndrome. J Appl Physiol 84:1311–1315
Buettner R, Schölmerich J, Bollheimer LC (2007) High-fat diet: modeling the metabolic disorders of human obesity in rodents. Obesity 15:798–808
Wallach S (1985) Clinical and biochemical aspects of chromium deficiency. J Am Coll Nutr 4:107–120
Becker DJ, Reul B, Ozeelikay A, (1996) Oral selenate improves glucose homeostasis and partly reverses abnormal expression of liver glycolytic and gluconeogenic enzymes in diabetic rats. Diabetologia 39:3–11
Faure P, Roussel A, Coudray C, Richard MJ, Halimi S, Favier A (1992) Zinc and insulin sensitivity. Biol Trace Elem Res. 32:305–310
Sahin K, Onderci M, Tuzcu M, (2007) Effect of chromium on carbohydrate and lipid metabolism in a rat model of type 2 diabetes mellitus: the fat-fed, streptozotocin-treated rat. Metabolism 569:1233–1240
Chen MD, Lin PY, Tsou CT, Wang JJ, Lin WH (1995) Selected metals status in patients with noninsulin dependent diabetes mellitus. Biol Trace Elem Res 50:119–124
Anderson RA. Chromium, glucose intolerance and diabetes (1998) J Am Coll Nutr 176:548–555
Feng W, Ding W, Qian Q, (1999) Comparison of the chromium distribution in organs and subcellular fractions of normal and diabetic rats by using enriched stable isotope Cr-50 tracer technique. Biol Trace Elem Res 71–72:121–129
Ding W, Chai Z, Duan P, (1998) Serum and urine chromium concentrations in elderly diabetics. Biol Trace Elem Res 633:231–237
Cefalu WT, Hu FB (2004) Role of chromium in human health and in diabetes. Diabetes Care 27:2741–2751
Jeejeebhoy KN, Chur RC, Mariss E, (1977) Chromium deficiency, glucose intolerance and neuropathy reversed by chromium supplementation in a patient receiving long-term total parenteral nutrition. Am J Clin Nutr 30:531–538
Bartlett HE, Eperjesi F (2008) Nutritional supplementation for type 2 diabetes: a systematic review. Ophthalmic Physiol Opt 286:503–523
Anderson RA (1987) Chromium, Trace Elements in Human and Animal Nutrition. pp. 225–244. Academic. New York.
Mertz W (1993) Chromium in human nutrition. A review. J Nutr 123, 626–630
Anderson RA, Polansky MM, Bryden NA (2004) Stability and absorption of chromium and absorption of chromium histidinate complexes by humans. Biol Trace Elem Res 1013:211–218
Sahin N, Sahin K, Erkal N (1999) The effect of chromium added into basal diet on serum total protein, urea, triglyceride, cholesterol, and serum and tissue chromium, zinc, copper levels in rabbits. T J Vet Anim Sci 23,109–113
Reed MJ, Meszaros K, Entes LJ, (2000) A new rat model of type 2 diabetes: the fat-fed, streptozotocin-treated rat. Metabolism 4911:1390–1394
SAS (1999) SAS® User’s Guide: Statistics, Version 7th. Statistical Analysis System Institute Inc., Cary, NC, USA
Feng W, Qian Q, Ding W, Chai Z (2001) Tissue contents and subcellular distribution of chromium and other trace metals in experimental diabetic rats after intravenous injection of Cr 50-enriched stable isotopic tracer solution. Metabolism. 5010:1168–1174
Althuis MD, Jordan NE, Ludington EA, Wittes JT (2002) Glucose and insulin responses to dietary chromium supplements: a meta-analysis. Am J Clin Nutr 76:148–155
Uusitupa MI, Mykkänen L, Siitonen O, (1992) Chromium supplementation in impaired glucose tolerance of elderly: effects on blood glucose, plasma insulin, C-peptide and lipid levels. Br J Nutr 681:209–16
Thomas VL, Gropper SS (1996) Effect of chromium nicotinic acid supplementation on selected cardiovascular disease risk factors. Biol Trace Elem Res 553:297–305
Elamri FA, Elsaltani HA, Dagadkin NN, (1994) Determination of trace elements in normal and diabetic whole blood by neutron activation analysis. Biol Trace Elem Res. 43–45:383–388
Mita Y, Ishihara K, Fukuchi Y, (2005) Supplementation with chromium picolinate recovers renal Cr concentration and improves carbohydrate metabolism and renal function in type 2 diabetic mice. Biol Trace Elem Res 1051–3:229–248.
Schrauzer, GN, Shrestha, KP, Molenaar, TB, Mead, S (1986) Effects of chromium supplementation on food energy utilization and the trace-element composition in the liver and heart of glucose-exposed young mice Biol Trace Elem Res 9: 79–87.
Anderson RA, Bryden NA, Polansky MM (1997) Lack of toxicity of chromium chloride and chromium picolinate in rats. J Am Coll Nutr. 163:273–279.
Kazi S, Ali SS, Kazi TG, Kazi GH (1999) Chromium: its role in diabetes and concentration in human scalp hair. Am Clin Lab 187:8.
Failla ML, Kiser RA (1981) Altered tissue content and cytosol distribution of trace metals in experimental diabetes. J Nutr 111:1900–1909
Reeves PG, Briske-Anderson M, Johnson L (1998) Physiologic concentration of zinc affect the kinetics of copper uptake and transport in the human intestinal cell model, Caco-2. J Nutr 128 10:1794–1801
Ensminger ME, Oldfield JE, Heinemann WW (1990) Feeds and Nutrition. pp. 108–110. Ensminger, USA
Clodfelder BJ, Gullick BM, Lukaski HC, Neggers Y, Vincent JB (2005) Oral administration of the biomimetic [Cr3O(O2CCH2CH3)6(H2O)3]+ increases insulin sensitivity and improves blood plasma variables in healthy and type 2 diabetic rats. J Biol Inorg Chem 102:119–130.
Schrauzer GN, Shrestha KP, Molenaar TB, Mead S (1986) Effects of chromium supplementation on food energy utilization and the trace element composition in the liver and heart of glucose-exposed young mice. Biol Trace Elem Res 9, 79–87
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We thank Nutrition 21, Purchase, NY, for providing chromium histidinate and financial support for this study.
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Dogukan, A., Sahin, N., Tuzcu, M. et al. The Effects of Chromium Histidinate on Mineral Status of Serum and Tissue in Fat-Fed and Streptozotocin-Treated Type II Diabetic Rats. Biol Trace Elem Res 131, 124–132 (2009). https://doi.org/10.1007/s12011-009-8351-8
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DOI: https://doi.org/10.1007/s12011-009-8351-8