Abstract
With a photocatalyzed characteristic, nanoanatase TiO2 under light could cause an oxidation–reduction reaction. Our studies had proved that nano-TiO2 could promote photosynthesis and greatly improve spinach growth. However, the mechanism of nano-TiO2 on promoting conversion from light energy to electron energy and from electron energy to active chemistry energy remains largely unclear. In this study, we report that the electron transfer, oxygen evolution, and photophosphorylation of chloroplast (Chl) from nanoanatase-TiO2-treated spinach were greatly increased under visible light and ultraviolet light illumination. It was demonstrated that nanoanatase TiO2 could greatly improve whole chain electron transport, photoreduction activity of photosystem II, O2-evolving and photophosphorylation activity of spinach Chl not only under visible light, but also energy-enriched electron from nanoanatase TiO2, which entered Chl under ultraviolet light and was transferred in photosynthetic electron transport chain and made NADP+ be reduced into NADPH, and coupled to photophosphorylation and made electron energy be transformed to ATP. Moreover, nanoanatase h+, which photogenerated electron holes, captured an electron from water, which accelerated water photolysis and O2 evolution.
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Acknowledgments
This work was supported by the National Natural Science Foundation of China (grant nos. 20671067 and 30470150) and by the Jiangsu Province Universities Natural Science Foundation (grant nos. 06KJB180094 and 03KJB180122).
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Lei, Z., Mingyu, S., Chao, L. et al. Effects of Nanoanatase TiO2 on Photosynthesis of Spinach Chloroplasts Under Different Light Illumination. Biol Trace Elem Res 119, 68–76 (2007). https://doi.org/10.1007/s12011-007-0047-3
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DOI: https://doi.org/10.1007/s12011-007-0047-3