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Effects of Nano-anatase TiO2 on Absorption, Distribution of Light, and Photoreduction Activities of Chloroplast Membrane of Spinach

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An Erratum to this article was published on 26 June 2009

Abstract

The effects of nano-anatase TiO2 on light absorption, distribution, and conversion, and photoreduction activities of spinach chloroplast were studied by spectroscopy. Several effects of nano-anatase TiO2 were observed: (1) the absorption peak intensity of the chloroplast was obviously increased in red and blue region, the ratio of the Soret band and Q band was higher than that of the control; (2) the great enhancement of fluorescence quantum yield near 680 nm of the chloroplast was observed, the quantum yield under excitation wavelength of 480 nm was higher than the excitation wavelength of 440 nm; (3) the excitation peak intensity near 440 and 480 nm of the chloroplast significantly rose under emission wavelength of 680 nm, and F 480 / F 440 ratio was reduced; (4) when emission wavelength was at 720 nm, the excitation peaks near 650 and 680 nm were obviously raised, and F 650 / F 680 ratio rose; (5) the rate of whole chain electron transport, photochemical activities of PSII DCPIP photoreduction and oxygen evolution were greatly improved, but the photoreduction activities of PSI were a little changed. Together, the studies of the experiments showed that nano-anatase TiO2 could increase absorption of light on spinach chloroplast and promote excitation energy to be absorbed by LHCII and transferred to PSII and improve excitation energy from PSI to be transferred to PSII, thus, promote the conversion from light energy to electron energy and accelerate electron transport, water photolysis, and oxygen evolution.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (grant nos. 20671067, 30470150) and by the Jiangsu Province Universities Natural Science Foundation (grant nos. 06KJB180094, 03KJB180122).

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Authors and Affiliations

  1. College of Life Sciences, Suzhou University, Suzhou, 215123, People’s Republic of China

    Su Mingyu, Hong Fashui, Liu Chao, Wu Xiao, Liu Xiaoqing, Chen Liang, Gao Fengqing & Yang Fan

  2. School of Chemical, Biological and Materials Engineering, University of Oklahoma, Norman, OK, 73019, USA

    Li Zhongrui

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  1. Su Mingyu
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  2. Hong Fashui
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  3. Liu Chao
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  4. Wu Xiao
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  7. Gao Fengqing
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  9. Li Zhongrui
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Correspondence to Hong Fashui.

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An erratum to this article is available at http://dx.doi.org/10.1007/s12011-009-8430-x.

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Mingyu, S., Fashui, H., Chao, L. et al. Effects of Nano-anatase TiO2 on Absorption, Distribution of Light, and Photoreduction Activities of Chloroplast Membrane of Spinach. Biol Trace Elem Res 118, 120–130 (2007). https://doi.org/10.1007/s12011-007-0006-z

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