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Evaluation of Bioactive Attributes and Emulsification Potential of Exopolysaccharide Produced by a Brown-rot Fungus Fomitopsis meliae AGDP-2

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Abstract

Mushrooms possess wide array of biologically active secondary metabolites and have been traditionally used for their medicinal properties. Exopolysaccharide (EPS) is one of such bioactive metabolites. The bioactive attributes and emulsification capabilities of the exopolysaccharides produced by a novel brown-rot fungus Fomitopsis meliae AGDP-2 under submerged fermentation has been thoroughly investigated in the present study. Exopolysaccharide displayed anti-oxidant activities in dose dependent manner with the maximum scavenging of ABTS radicals (42.45%), DPPH radicals (75.34%), Hydroxyl radicals (63.64%), Superoxide anion radical (76.54%) and Ferric Reducing Antioxidant Power with IC50 value of 231 µg/mL. Additionally, evaluation of anti-proliferative properties revealed that EPS significantly inhibited the proliferation of HepG2 and HT-29 cancer cells followed by moderate inhibition of HeLa and MCF-7 cancer cell lines and quite less inhibition of L-132 and KB cell lines. The IC50 values of EPS for the abovementioned cell lines are 9.465 µg/mL, 11.25 µg/mL, 38.98 µg/mL, 87.78 µg/mL, 2061 µg/mL and 2361 µg/mL respectively. Moreover EPS also possess good anti-microbial as well as anti-biofilm properties. The studies on emulsification potential described that EPS is good emulsifier of different vegetable oils and the emulsion formed was quite stable up to 144 h.

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Acknowledgements

Authors wish to acknowledge Sophisticated Instrumentation Centre for Applied Research and Testing – SICART and SAIF, IIT Bombay for providing instrumentation facility.

Funding

This study was financially supported by the University Grants Commission, New Delhi; by providing NFOBC fellowship (Student ID: 201819-NFO-2018-19-OBC-GUJ-69853). Author DP has received this research support.

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  1. Department of Microbiology, Natubhai V. Patel College of Pure and Applied Sciences, Near Post Office, Mota Bazar, Vallabh Vidyanagar, 388120, Gujarat, India

    Darshankumar Prajapati, Ashish Bhatt & Akshaya Gupte

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DP was responsible for conceptualization, methodology, acquisition of data, analysis, interpretation of data, and original drafting of manuscript; AB for material preparation, data collection, software and analysis; AG for critical revision of the manuscript and intellectual inputs and supervision of the study; All authors read and approved final manuscript.

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Correspondence to Akshaya Gupte.

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Prajapati, D., Bhatt, A. & Gupte, A. Evaluation of Bioactive Attributes and Emulsification Potential of Exopolysaccharide Produced by a Brown-rot Fungus Fomitopsis meliae AGDP-2. Appl Biochem Biotechnol 195, 2974–2992 (2023). https://doi.org/10.1007/s12010-022-04257-0

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