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Potential Bioactive Compounds from Marine Streptomyces sp. and Their In Vitro Antibiofilm and Antibacterial Activities Against Antimicrobial-Resistant Clinical Pathogens

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Abstract

Antimicrobial resistance issues have risen dramatically in recent years, posing a severe concern to humans worldwide. The urgent need to find novel compounds for pharmaceutical applications prompts the research of under-explored environments such as marine ecosystems. The present study was designed to discover novel secondary metabolites, and we have isolated about 30 actinomycetes from the marine soil samples collected in Thondi (Ramanathapuram, Tamil Nadu, India), where most isolates are associated with the genus Streptomyces. Out of 30, one potentially active strain (Streptomyces sp. SRMA3) was identified using primary and secondary screening methods against the drug-resistant clinical pathogens. The active metabolites extracted from the selected active isolate were subjected to partial purification and characterization using Fourier transform infrared spectrophotometer (FTIR) and gas chromatography-mass spectroscopy (GC-MS) analysis. The minimum inhibitory concentration (MIC) value was determined for the active metabolite. Further, the partially purified active fraction was revealed for its antibacterial and antibiofilm activity against drug-resistant clinical pathogens. Light and fluorescence microscopy detected the viability and adhesion of the biofilm-forming drug-resistant pathogens. Growth curve analysis showed that the active metabolite has the potential to inhibit drug-resistant pathogens. The synergistic effect of active metabolite with commercial antibiotics also revealed that it could enhance the activity of antibiotics in antimicrobial resistance pathogens. This study shows that the isolated Streptomyces sp. SRMA3 is a potentially active strain, and the metabolite derived from this strain has a good antibacterial and antibiofilm activity against antimicrobially resistant clinical pathogens and could be used for various biotechnological applications.

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Acknowledgements

The authors are grateful to Medical Microbiology Laboratory Unit at Department of Microbiology, Alagappa University, for providing research facilities to carry out this research work.

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Authors and Affiliations

  1. Medical Microbiology Laboratory, Department of Microbiology, Alagappa University, Karaikudi, Tamil Nadu, 630008, India

    Ranjithkumar Dhandapani & Sathiamoorthi Thangavelu

  2. Research and Development Division, Chimertech Private Limited, Chennai, India

    Ranjithkumar Dhandapani & Ragul Paramasivam

  3. Department of Microbiology, Aarupadai Veedu Medical College and Hospital, Pondicherry, 607402, India

    Latha Ragunathan

  4. Centre for Materials Engineering and Regenerative medicine, Bharath Institute of Higher Education and Research, Chennai, Tamil Nadu, India

    Palanivel Velmurugan

  5. AMR and Nanotherapeutic Laboratory, Department of Pharmacology, Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences (SIMATS), Chennai, Tamil Nadu, 600077, India

    Saravanan Muthupandian

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  1. Ranjithkumar Dhandapani
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  2. Sathiamoorthi Thangavelu
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  3. Latha Ragunathan
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  6. Saravanan Muthupandian
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Contributions

Conceptualization, writing original draft, writing review and editing, methodology, project administration, software, visualization, data curation, resources: Ranjithkumar Dhandapani; validation: Ragul Paramasivam, Palanivel Velmurugan; formal analysis: Latha Ragunathan; supervision: Sathiamoorthi Thangavelu, and Saravanan Muthupandian

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Correspondence to Sathiamoorthi Thangavelu or Saravanan Muthupandian.

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Dhandapani, R., Thangavelu, S., Ragunathan, L. et al. Potential Bioactive Compounds from Marine Streptomyces sp. and Their In Vitro Antibiofilm and Antibacterial Activities Against Antimicrobial-Resistant Clinical Pathogens. Appl Biochem Biotechnol 194, 4702–4723 (2022). https://doi.org/10.1007/s12010-022-04072-7

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