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Enhanced Biosynthesis of D-Arabitol by Metschnikowia reukaufii Through Optimizing Medium Composition and Fermentation Conditions

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Abstract

D-Arabitol is an important functional sugar alcohol, which can be used in the preparation of foods, chemicals, and medicines. Despite biological production of D-arabitol from low-cost substrates has recently been the focus of research, low yield of this technology has limited its large-scale exploitation. Optimization of this bioprocess could be a promising option to improve the yield of D-arabitol. In this study, one-factor-at-a-time (OFAT) strategy and Box-Behnken design (BBD) were used to increase D-arabitol production by Metschnikowia reukaufii CICC 31,858 through optimizing the fermentation conditions and medium composition. The OFAT optimization provided the optimal conditions for temperature, agitation speed, and fermentation time of 30℃, 220 rpm, and 6 days, respectively. Likewise, the optimum concentrations of peptone, ammonium sulfate, KH2PO4, MgSO4·7H2O, and fumaric acid in the fermentation medium were (g/L) 7.5, 1, 2, 0.5, and 7.5, respectively. Under these optimum conditions, 80.43 g/L of D-arabitol was produced from 200 g/L of glucose, with a productivity of 0.56 g/L/h. The BBD optimization with three important components of fermentation medium (KH2PO4, MgSO4·7H2O, and fumaric acid) showed that the predicted titer of D-arabitol varied from 47.21 to 89.27 g/L, and the actual titer of D-arabitol ranged from 47.36 to 89.83 g/L. The optimum concentrations (g/L) of KH2PO4, MgSO4·7H2O, and fumaric acid in the fermentation medium were found to be 1.0, 0.5, and 4.7 g/L, respectively. Under the optimum conditions, 92.45 g/L of D-arabitol was finally produced with the yield and productivity of 0.46 g/g and 0.64 g/L/h, respectively.

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Data availability

The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.

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Funding

This work was supported by the National Natural Science Foundation of China (Grant No. 31972042, 32150410349), National Key R & D Program of China (Grant No. 2021YFA0910401), Natural Science Foundation of Jiangsu Province (Grant No. BK20210752), China Postdoctoral Science Foundation (Grant No. 2019M661759), Foreign Experts Program of Ministry of Science and Technology of China (Grant No. QNJ20200214015), and Innovation and Entrepreneurship Program of Jiangsu Province (Grant No. JSSCBS20210929).

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  1. Jiaqi Huang and Yingfeng An contributed equally to this work.

Authors and Affiliations

  1. School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu, 212013, China

    Jiaqi Huang, Hossain M. Zabed, Yuvaraj Ravikumar, Mei Zhao, Junhua Yun, Guoyan Zhang, Yufei Zhang, Xiaolan Li & Xianghui Qi

  2. College of Biosciences and Biotechnology, Shenyang Agricultural University, 120 Dongling Road, Shenyang, Liaoning, 110161, China

    Yingfeng An

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Contributions

Jiaqi Huang: Conceptualization, investigation, formal analysis, writing—original draft. Yingfeng An: Investigation, formal analysis, writing—review and editing. Hossain M Zabed: Conceptualization, writing—review and editing. Yuvaraj Ravikumar: Writing—review and editing. Mei Zhao: Writing—review and editing. Junhua Yun: Formal analysis. Guoyan Zhang: Resources, formal analysis. Yufei Zhang: Resources, formal analysis. Xiaolan Li: Investigation, formal analysis. Xianghui Qi: Conceptualization; writing, review and editing; supervision.

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Correspondence to Xianghui Qi.

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Huang, J., An, Y., Zabed, H.M. et al. Enhanced Biosynthesis of D-Arabitol by Metschnikowia reukaufii Through Optimizing Medium Composition and Fermentation Conditions. Appl Biochem Biotechnol 194, 3119–3135 (2022). https://doi.org/10.1007/s12010-022-03910-y

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