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Anti-Diabetic Activity of Silver Nanoparticles Synthesized from the Hydroethanolic Extract of Myristica fragrans Seeds

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Abstract

Myristica fragrans, also known as nutmeg, is a spice that cures various diseases. This study aimed to synthesize silver nanoparticles from a hydroethanolic extract of Myristica fragrans seeds (MFHE) and evaluate their anti-diabetic properties. To MFHE, AgNO3 solution was added and exposed to sunlight to produce silver nanoparticles from hydroethanolic seed extract of Myristica fragrans (MFHENP). The MFHENP was characterized by numerous techniques. UV-visible spectroscopy confirmed the formation of silver nanoparticles by the absorption peak at 430nm. Scanning electron microscopy (SEM) studies revealed the shape and size of the particles at the range of 50–60nm. Energy-dispersive X-ray spectroscopy (EDX) disclosed the presence of silver ions. X-ray diffraction spectrum confirmed the crystalline nature of silver nanoparticles by the peak at 39°. FTIR analysis revealed the functional groups present in MFHE as well as in MFHENP and zeta potential analysis was found to be 14mV. Furthermore, in vitro anti-diabetic activity was investigated. MFHENP showed significant efficiency against the inhibition of alpha-amylase and alpha-glucosidase enzymes and also MFHENP retarded the glucose transport across the membrane which is analyzed by glucose diffusion and glucose uptake assays. Acarbose is used as a standard for all these methods and MFHENP efficiency proves their therapeutic potential for the treatment of diabetes mellitus.

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All data generated or analyzed during this study are included in this published article [and its supplementary information files].

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

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  1. Department of Biochemistry, PSG College of Arts & Science, Coimbatore, Tamil Nadu, 641014, India

    Ramya Perumalsamy & Lavanya Krishnadhas

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  1. Ramya Perumalsamy
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  2. Lavanya Krishnadhas
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RP contributed to the data collection, data analysis and interpretation, article drafting, and critical revision of the article. LK contributed to the design of work and article finalization.

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Correspondence to Ramya Perumalsamy.

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Perumalsamy, R., Krishnadhas, L. Anti-Diabetic Activity of Silver Nanoparticles Synthesized from the Hydroethanolic Extract of Myristica fragrans Seeds. Appl Biochem Biotechnol 194, 1136–1148 (2022). https://doi.org/10.1007/s12010-022-03825-8

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  • DOI: https://doi.org/10.1007/s12010-022-03825-8

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