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Patterns of Lignocellulosic Sugar Assimilation and Lipid Production by Newly Isolated Yeast Strains From Chilean Valdivian Forest

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Abstract

Three yeast strains were isolated from decaying wood of Chilean Valdivian forest and identified as Meyerozyma guilliermondii, Scheffersomyces coipomensis, and Sugiyamaella paludigena. These strains were able to efficiently grow on the major monomers contained in Pinus spp. and Eucalyptus spp. wood that includes glucose (Glc), xylose (Xyl), and mannose (Man), showing at 28 °C higher uptake rates for Man, and in some cases for Glc, than for Xyl, used as single carbon sources. Nevertheless, in cultures performed on sugar mixtures, the strains displayed a notable preference for Glc. Additionally, in sugar mixtures, the absence of regulatory mechanisms in sugar assimilation (e.g., catabolic repression) was observed and documented when the activities of several enzymes involved in sugar assimilation (i.e., phosphoglucose isomerase, phosphomannose isomerase, and xylulokinase) were determined. The activity of the key enzymes involved in the onset of lipid accumulation (i.e., NAD+-ICDH) and in fatty acid (FA) biosynthesis (i.e., ATP:CL) indicated a significant accumulation of storage lipids (i.e., up to 24%, w/w) containing oleic and palmitic acids as the major components. The present paper is the first report on the potential of M. guilliermondii, S. coipomensis, and S. paludigena as oleaginous yeasts. We conclude that the new isolates, being able to simultaneously assimilate the major lignocellulosic sugars and efficiently convert them into oily biomass, present a biotechnological potential which deserve further investigation.

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Abbreviations

ATP:CL :

ATP-citrate lyase

FA:

Fatty acid

Glc :

Glucose

Man :

Mannose

NAD+-ICDH :

NAD+-dependent isocitrate dehydrogenase

PGI :

Phosphoglucose isomerase

PMI :

Phosphomannose isomerase

PUFA:

Polyunsaturated fatty acid

SCO:

Single cell oil

TCA:

Tricarboxylic acid

XK :

Xylulokinase

Xyl :

Xylose

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Acknowledgments

The authors would like to thank Marianna Dourou for technical assistance.

Funding

The project was financially supported by the CONICYT PFCHA/DOCTORADO BECAS CHILE/2018-21180068, the University of Patras, Greece, and the Universidad de Concepción, Chile.

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Authors and Affiliations

  1. Department of Biology, Division of Genetics, Cell Biology and Development, University of Patras, Patras, Greece

    Gabriela Valdés & George Aggelis

  2. Centro de Biotecnología, Universidad de Concepción, Concepción, Chile

    Gabriela Valdés, Regis Teixeira Mendonça & Carolina Parra

  3. Facultad de Ciencias Forestales, Universidad de Concepción, Concepción, Chile

    Gabriela Valdés & Regis Teixeira Mendonça

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  1. Gabriela Valdés
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  2. Regis Teixeira Mendonça
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Correspondence to George Aggelis.

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Valdés, G., Mendonça, R.T., Parra, C. et al. Patterns of Lignocellulosic Sugar Assimilation and Lipid Production by Newly Isolated Yeast Strains From Chilean Valdivian Forest. Appl Biochem Biotechnol 192, 1124–1146 (2020). https://doi.org/10.1007/s12010-020-03398-4

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