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Effect of Substrate to Inoculum Ratio on Biogas Production and Microbial Community During Hemi-Solid-State Batch Anaerobic Co-digestion of Rape Straw and Dairy Manure

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Abstract

The substrate to inoculum (S/I) ratio is crucial for the rapid start-up of solid-state anaerobic digestion (SS-AD) systems. In this study, the performance of methane production and microbial community structure were evaluated during co-digestion of rape straw (RS) and dairy manure (DM) at different S/I ratios (2:3, 1:1, 2:1, 3:1, and 4:1) in batch hemi-solid-state anaerobic digestion (HSS-AD) tests. The highest methane yield of 209.1 mL/g VSadded and highest volumetric methane production of 0.4 L/(L·d) were achieved at S/I ratios of 2:3 and 2:1, respectively. Lower S/I ratios (1:2, 1:1, and 2:1) steadily produced biogas throughout the AD period, while higher S/I ratios (3:1 and 4:1) failed to produce biogas during the initial stage of AD because of excess accumulation of volatile fatty acids and low pH. The predominant bacteria and archaea in stable digesters were Firmicutes and acetoclastic Methanosaeta, respectively, while Bacteroidetes predominated and the relative abundance of hydrogenotrophic Methanobacterium increased significantly in acidic digesters. Amounts of bacteria and archaea were inhibited in acidic digesters. Our results provide useful information for enhancing efficient methane production and advancing the understanding of the microbiome in HSS-AD of RS and DM at different S/I ratios.

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Funding

This work was supported by the National Natural Science Foundation of China (No. 51508258), the Sichuan Science and Technology Department Program of China (No. 2017JY0175), the Key Laboratory of Development and Application of Rural Renewable Energy Program, Ministry of Agriculture, China (No. 2015012), and the Scientific Research Foundation of Leshan Normal University (No. Z1410).

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Authors and Affiliations

  1. College of Chemistry, Leshan Normal University, Leshan, 614004, Sichuan, China

    Xuguang Ma, Tao Jiang, Jiali Chang & Qiong Tang

  2. Key Laboratory of Development and Application of Rural Renewable Energy, Biogas Institute of Ministry of Agriculture, Chengdu, 610041, China

    Tao Luo

  3. College of Agronomy and Biotechnology, Center of Biomass Engineering, China Agricultural University, Beijing, 100193, China

    Zongjun Cui

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  1. Xuguang Ma
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Correspondence to Xuguang Ma.

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Ma, X., Jiang, T., Chang, J. et al. Effect of Substrate to Inoculum Ratio on Biogas Production and Microbial Community During Hemi-Solid-State Batch Anaerobic Co-digestion of Rape Straw and Dairy Manure. Appl Biochem Biotechnol 189, 884–902 (2019). https://doi.org/10.1007/s12010-019-03035-9

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