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Arcopilus aureus, a Resveratrol-Producing Endophyte from Vitis vinifera

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Abstract

Resveratrol is extensively being used as a therapeutic moiety, as well as a pharmacophore for development of new drugs due to its multifarious beneficial effects. The objective of the present study was to isolate and screen the resveratrol-producing endophytic fungi from different varieties of Vitis vinifera. A total of 53 endophytic fungi belonging to different fungal genera were isolated from the stem and leaf tissues of Vitis vinifera (merlot, wild, pinot noir, Shiraz, muscat) from different grape-producing locations of India. Only 29 endophytic fungal isolates exhibited a positive test for phenolics by phytochemical methods. The resveratrol obtained after ethyl acetate extraction was confirmed using standard molecule on thin layer chromatography (TLC) with a retention factor (Rf) of 0.69. The purified and standard resveratrol were visualized under UV light as a violet-colored spot. In HPLC analysis of the ethyl acetate extract of culture broth of 11 endophytic isolates, the highest resveratrol content was found in #12VVLPM (89.1 μg/ml) followed by #18VVLPM (37.3 μg/ml) and 193VVSTPM (25.2 μg/ml) exhibiting a retention time of 3.36 min which corresponded to the standard resveratrol. The resveratrol-producing isolates belong to seven genera viz. Aspergillus, Botryosphaeria, Penicillium, Fusarium, Alternaria, Arcopilus, and Lasiodiplodia, and using morphological and molecular methods, #12VVLPM was identified as Arcopilus aureus.

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Acknowledgments

The authors thank the Department of Biotechnology and TIFAC-CORE (Centre of Relevance and Excellence), Thapar Institute of Engineering & Technology, Patiala, Punjab, for providing the necessary infrastructure to carry out the research work.

Funding

The authors thank the Department of Biotechnology (DBT), Government of India, New Delhi, for financial assistance through Project No. BT/PR9094/NDB/39/378/2013

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  1. Department of Biotechnology, Thapar Institute of Engineering and Technology, Patiala, Punjab, 147004, India

    Vagish Dwibedi & Sanjai Saxena

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  1. Vagish Dwibedi
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  2. Sanjai Saxena
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Correspondence to Sanjai Saxena.

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Dwibedi, V., Saxena, S. Arcopilus aureus, a Resveratrol-Producing Endophyte from Vitis vinifera. Appl Biochem Biotechnol 186, 476–495 (2018). https://doi.org/10.1007/s12010-018-2755-x

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