Abstract
The reuse of waste as well as the production of biodegradable compounds has for years been the object of studies and of global interest as a way to reduce the environmental impact generated by unsustainable exploratory processes. The conversion of linear processes into cyclical processes has environmental and economic advantages, reducing waste deposition and reducing costs. The objective of this work was to use biopolymer extraction waste in the cultivation of Spirulina sp. LEB 18, for the cyclic process of polyhydroxybutyrate (PHB) synthesis. Concentrations of 10, 15, 20, 25, and 30% (v/v) of biopolymer extraction waste were tested. For comparison, two assays were used without addition of waste, Zarrouk (SZ) and modified Zarrouk (ZM), with reduction of nitrogen. The assays were carried out in triplicate and evaluated for the production of microalgal biomass and PHB. The tests with addition of waste presented a biomass production statistically equal to ZM (0.79 g L−1) (p < 0.1). The production of PHB in the assay containing 25% of waste was higher when compared to the other cultivations, obtaining 10.6% (w/w) of biopolymer. From the results obtained, it is affirmed that the use of PHB extraction waste in the microalgal cultivation, aiming at the synthesis of biopolymers, can occur in a cyclic process, reducing process costs and the deposition of waste, thus favoring the preservation of the environment.
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References
Pauli, G. (2014). A economia azul: 10 anos, 100 inovações, 100000000 de empregos (1st ed.). Brookline: Paradigm Pubns.
Vonshak, A. (1997). Spirulina platensis (Arthrospira): physiology, cell-biology, and biotechnology. Abingdon: Taylor & Francis.
Singh, A. K., Sharma, L., Mallick, N., & Mala, J. (2017). Progress and challenges in producing polyhydroxyalkanoate biopolymers from cyanobacteria. Journal of Applied Phycology, 29(3), 1213–1232. https://doi.org/10.1007/s10811-016-1006-1.
Pandey, A., Lee, D.-J., Chisti, Y., & Soccol, C. R. (2014). Biofuels from algae. San Diego: Elsevier B.V.
Troschl, C., Meixner, K., & Drosg, B. (2017). Cyanobacterial PHA production—review of recent advances and a summary of three years’ working experience running a pilot plant. Bioengineering, 4(2), 26. https://doi.org/10.3390/bioengineering4020026.
Fradinho, J. C., Oehmen, A., & Reis, M. A. M. (2014). Photosynthetic mixed culture polyhydroxyalkanoate (PHA) production from individual and mixed volatile fatty acids (VFAs): Substrate preferences and co-substrate uptake. Journal of Biotechnology, 185, 19–27. https://doi.org/10.1016/j.jbiotec.2014.05.035.
Markou, G., Chatzipavlidis, I., & Georgakakis, D. (2012). Cultivation of Arthrospira (Spirulina) platensis in olive-oil mill wastewater treated with sodium hypochlorite. Bioresource Technology, 112, 234–241. https://doi.org/10.1016/j.biortech.2012.02.098.
Abdel-Raouf, N., Al-Homaidan, A. A., & Ibraheem, I. B. M. (2012). Microalgae and wastewater treatment. Saudi journal of biological sciences, 19(3), 257–275. https://doi.org/10.1016/j.sjbs.2012.04.005.
da Silva Vaz, B., Costa, J. A. V., & Morais, M. G. (2016). CO2 biofixation by the cyanobacterium Spirulina sp. LEB 18 and the green alga Chlorella fusca LEB 111 grown using gas effluents and solid residues of thermoelectric origin. Applied Biochemistry and Biotechnology, 178(2), 418–429. https://doi.org/10.1007/s12010-015-1876-8.
Singh, R., Bhaskar, T., & Balagurumurthy, B. (2014). Hydrothermal upgradation of algae into value-added hydrocarbons. Biofuels from Algae (pp. 235–260). Amsterdam: Elsevier. https://doi.org/10.1016/B978-0-444-59558-4.00011-5.
Ho, S.-H., Huang, S.-W., Chen, C.-Y., Hasunuma, T., Kondo, A., & Chang, J.-S. (2013). Bioethanol production using carbohydrate-rich microalgae biomass as feedstock. Bioresource Technology, 135, 191–198. https://doi.org/10.1016/j.biortech.2012.10.015.
Laycock, B., Halley, P., Pratt, S., Werker, A., & Lant, P. (2013). The chemomechanical properties of microbial polyhydroxyalkanoates. Progress in Polymer Science, 39(2), 397–442. https://doi.org/10.1016/j.progpolymsci.2013.06.008.
Fabra, M. J., López-Rubio, A., & Lagaron, J. M. (2015). Three-layer films based on wheat gluten and electrospun PHA. Food and Bioprocess Technology, 8(11), 2330–2340. https://doi.org/10.1007/s11947-015-1590-0.
Fan, X., Jiang, Q., Sun, Z., Li, G., Ren, X., Liang, J., & Huang, T. S. (2015). Preparation and characterization of electrospun antimicrobial fibrous membranes based on polyhydroxybutyrate (PHB). Fibers and Polymers, 16(8), 1751–1758. https://doi.org/10.1007/s12221-015-5108-1.
Park, J., Jin, H.-F., Lim, B.-R., Park, K.-Y., & Lee, K. (2010). Ammonia removal from anaerobic digestion effluent of livestock waste using green alga Scenedesmus sp. Bioresource Technology, 101(22), 8649–8657. https://doi.org/10.1016/j.biortech.2010.06.142.
Martins, R. G., Gonçalves, I. S., De Morais, M. G., & Costa, J. A. V. (2014). Bioprocess engineering aspects of biopolymer production by the cyanobacterium Spirulina strain LEB 18. International Journal of Polymer Science, 2014, 1–7. https://doi.org/10.1155/2014/895237.
Coelho, V. C., Silva, C. K., Terra, A. L., Costa, J. A. V., & De Morais, M. G. (2015). Polyhydroxybutyrate production by Spirulina sp. LEB 18 grown under different nutrient concentrations. African Journal of Microbiology Research, 9(24), 1586–1594. https://doi.org/10.5897/AJMR2015.7530.
Pelizer, L. H., Danesi, E. D. G., de O Rangel, C., Sassano, C. E., Carvalho, J. C. M., Sato, S., & Moraes, I. O. (2003). Influence of inoculum age and concentration in Spirulina platensis cultivation. Journal of Food Engineering, 56(4), 371–375. https://doi.org/10.1016/S0260-8774(02)00209-1.
Morais, M. G., & Costa, J. A. V. (2007). Isolation and selection of microalgae from coal fired thermoelectric power plant for biofixation of carbon dioxide. Energy Conversion and Management, 48(7), 2169–2173. https://doi.org/10.1016/j.enconman.2006.12.011.
Zarrouk, C. (1966). Contribution à l’étude d’une cyanophycée influence de divers facteurs physiques et chimiques sur la croissance et la photosynthese de Spirulina maxima (Setch et Gardner) Geitler. Paris: University of Paris.
Reichert, C. C., Reinehr, C. O., & Costa, J. A. V. (2006). Semicontinuous cultivation of the cyanobacterium Spirulina platensis in a closed photobioreactor. Brazilian Journal of Chemical Engineering, 23(1), 23–28. https://doi.org/10.1590/S0104-66322006000100003.
Carmouze, J. P. (1994). O metabolismo dos ecossistemas aquáticos: fundamentos teóricos, métodos de estudo e análises químicas. São Paulo: Edgard Blücher LTDA.
Cataldo, D. A., Maroon, M., Schrader, L. E., & Youngs, V. L. (2008). Rapid colorimetric determination of nitrate in plant tissue by nitration of salicylic acid 1. Communications in Soil Science and Plant Analysis, 6(1), 71–80. https://doi.org/10.1080/00103627509366547.
Bailey, J. E., & Ollis, D. F. (1986). Biochemical engineering fundamentals (2nd ed.). Michigan: McGraw-Hill.
Schimidell, W., de A Lima, U., Aquarone, E., & Borzani, W. (2001). Biotecnologia Industrial. São Paulo: Blucher.
Brandl, H., Gross, R. A., Lenz, R. W., & Fuller, R. C. (1988). Pseudomonas oleovorans as a source of poly(beta-hydroxyalkanoates) for potential applications as biodegradable polyesters. Applied and Environmental Microbiology, 54(8), 1977–1982 Retrieved from http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=202789&tool=pmcentrez&rendertype=abstract.
Zhang, Y.-Z., Liu, G.-M., Weng, W.-Q., Ding, J.-Y., & Liu, S.-J. (2015). Engineering of Ralstonia eutropha for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from glucose. Journal of Biotechnology, 195, 82–88. https://doi.org/10.1016/j.jbiotec.2014.12.014.
Lafferty, R. M., & Heinzle, E. (1978). Cyclic carbonic acid esters as solvents for poly-(β-hydroxybutyric acid. Canadian: Google Patents. Retrieved from http://www.google.com/patents/US4101533
Kumar, A., Ergas, S., Yuan, X., Sahu, A., Zhang, Q., Dewulf, J., et al. (2010). Enhanced CO2 fixation and biofuel production via microalgae: recent developments and future directions. Trends in Biotechnology, 28(7), 371–380. https://doi.org/10.1016/j.tibtech.2010.04.004.
Hille, R., Fagan, M., Bromfield, L., & Pott, R. (2013). A modified pH drift assay for inorganic carbon accumulation and external carbonic anhydrase activity in microalgae. Journal of Applied Phycology, 26(1), 377–385. https://doi.org/10.1007/s10811-013-0076-6.
Carvalho, L. F., Oliveira, M. S., & Alberto, J. C. V. (2014). Evaluation of the influence of nitrogen and phosphorus nutrients in the culture and production of biosurfactants by microalga Spirulina. Int. Journal of Engineering Research and Applications, 4(6), 90–98.
Richmond, A. (Ed.). (2007). Handbook of microalgal culture: biotechnology and applied phycology. Oxford: Blackwell Publishing Ltd. https://doi.org/10.1002/9780470995280.
Clarke, K. G. (2013). Bioprocess engineering, an introductory engineering and life science approach (1st ed.). Philadelphia: Woodhead Publishing Limited.
Allen, M. M., & Smith, A. J. (1969). Nitrogen chlorosis in blue-green algae. Archiv für Mikrobiologie, 69(2), 114–120. https://doi.org/10.1007/BF00409755.
Michelon, W., Da Silva, M. L. B., Mezzari, M. P., Pirolli, M., Prandini, J. M., & Soares, H. M. (2016). Effects of nitrogen and phosphorus on biochemical composition of microalgae polyculture harvested from phycoremediation of piggery wastewater digestate. Applied Biochemistry and Biotechnology, 178(7), 1407–1419. https://doi.org/10.1007/s12010-015-1955-x.
Jau, M. H., Yew, S. P., Toh, P. S. Y., Chong, A. S. C., Chu, W. L., Phang, S. M., et al. (2005). Biosynthesis and mobilization of poly(3-hydroxybutyrate) [P(3HB)] by Spirulina platensis. International Journal of Biological Macromolecules, 36(3), 144–151. https://doi.org/10.1016/j.ijbiomac.2005.05.002.
Singh, A. K., & Mallick, N. (2017). Advances in cyanobacterial polyhydroxyalkanoates production. FEMS Microbiology Letters, 364(20), 1–13. https://doi.org/10.1093/femsle/fnx189.
Hong, K., Sun, S., Tian, W., Chen, G. Q., & Huang, W. (1999). A rapid method for detecting bacterial polyhydroxyalkanoates in intact cells by Fourier transform infrared spectroscopy. Applied Microbiology and Biotechnology, 51(4), 523–526. https://doi.org/10.1007/s002530051427.
Kansiz, M., Billman-Jacobe, H., & McNaughton, D. (2000). Quantitative determination of the biodegradable polymer poly(beta-hydroxybutyrate) in a recombinant Escherichia coli strain by use of mid-infrared spectroscopy and multivariative statistics. Applied and Environmental Microbiology, 66(8), 3415–3420. https://doi.org/10.1128/AEM.66.8.3415-3420.2000.
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da Silva, C.K., Costa, J.A.V. & de Morais, M.G. Polyhydroxybutyrate (PHB) Synthesis by Spirulina sp. LEB 18 Using Biopolymer Extraction Waste. Appl Biochem Biotechnol 185, 822–833 (2018). https://doi.org/10.1007/s12010-017-2687-x
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DOI: https://doi.org/10.1007/s12010-017-2687-x